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Issue 1, 2013
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Gobichelin A and B: mixed-ligand siderophores discovered using proteomics

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Abstract

“Omic” strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.

Graphical abstract: Gobichelin A and B: mixed-ligand siderophores discovered using proteomics

  • This article is part of the themed collection: New Talent
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Publication details

The article was received on 07 Aug 2012, accepted on 18 Sep 2012 and first published on 24 Sep 2012


Article type: Concise Article
DOI: 10.1039/C2MD20232H
Citation: Med. Chem. Commun., 2013,4, 233-238
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    Gobichelin A and B: mixed-ligand siderophores discovered using proteomics

    Y. Chen, M. Unger, I. Ntai, R. A. McClure, J. C. Albright, R. J. Thomson and N. L. Kelleher, Med. Chem. Commun., 2013, 4, 233
    DOI: 10.1039/C2MD20232H

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