Issue 2, 2012

Microfabricated multiple field of view imaging flow cytometry

Abstract

The combination of microscopy and flow cytometry enables image based screening of large collections of cells. Despite the proposition more than thirty years ago, adding high resolution wide-field imaging to flow cytometers remains challenging. The velocity of cells in flow cytometry can surpass a meter per second, requiring either sub-microsecond exposure times or other sophisticated photodetection techniques. Instead of faster detectors and brighter sources, we demonstrate that by imaging multiple channels simultaneously, a high throughput can be maintained with a flow velocity reduced in proportion to the degree of parallelization. The multi-field of view imaging flow cytometer (MIFC) is implemented with parallel arrays of microfluidic channels and diffractive lenses that produce sixteen wide field images with a magnification of 45 and submicron resolution. Using this device, we have imaged latex beads, red blood cells, and acute myeloid leukemia cells at rates of 2,000–20,000 per second.

Graphical abstract: Microfabricated multiple field of view imaging flow cytometry

Article information

Article type
Paper
Submitted
02 Sep 2011
Accepted
12 Oct 2011
First published
31 Oct 2011

Lab Chip, 2012,12, 268-273

Microfabricated multiple field of view imaging flow cytometry

E. Schonbrun, S. S. Gorthi and D. Schaak, Lab Chip, 2012, 12, 268 DOI: 10.1039/C1LC20843H

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