Jump to main content
Jump to site search

Issue 2, 2012
Previous Article Next Article

Microfabricated multiple field of view imaging flow cytometry

Author affiliations


The combination of microscopy and flow cytometry enables image based screening of large collections of cells. Despite the proposition more than thirty years ago, adding high resolution wide-field imaging to flow cytometers remains challenging. The velocity of cells in flow cytometry can surpass a meter per second, requiring either sub-microsecond exposure times or other sophisticated photodetection techniques. Instead of faster detectors and brighter sources, we demonstrate that by imaging multiple channels simultaneously, a high throughput can be maintained with a flow velocity reduced in proportion to the degree of parallelization. The multi-field of view imaging flow cytometer (MIFC) is implemented with parallel arrays of microfluidic channels and diffractive lenses that produce sixteen wide field images with a magnification of 45 and submicron resolution. Using this device, we have imaged latex beads, red blood cells, and acute myeloid leukemia cells at rates of 2,000–20,000 per second.

Graphical abstract: Microfabricated multiple field of view imaging flow cytometry

Back to tab navigation

Publication details

The article was received on 02 Sep 2011, accepted on 12 Oct 2011 and first published on 31 Oct 2011

Article type: Paper
DOI: 10.1039/C1LC20843H
Citation: Lab Chip, 2012,12, 268-273
  •   Request permissions

    Microfabricated multiple field of view imaging flow cytometry

    E. Schonbrun, S. S. Gorthi and D. Schaak, Lab Chip, 2012, 12, 268
    DOI: 10.1039/C1LC20843H

Search articles by author