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Issue 20, 2012
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Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

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Abstract

Although polymerization-based amplification (PBA) has demonstrated promise as an inexpensive technique for use in molecular diagnostics, oxygen inhibition of radical photopolymerization has hindered its implementation in point-of-care devices. The addition of 0.3–0.7 μM eosin to an aqueous acrylate monomer solution containing a tertiary amine allows an interfacial polymerization reaction to proceed in air only near regions of a test surface where additional eosin initiators coupled to proteins have been localized as a function of molecular recognition events. The dose of light required for the reaction is inversely related to eosin concentration. This system achieves sensitivities comparable to those reported for inert gas-purged systems and requires significantly shorter reaction times. We provide several comparisons of this system with other implementations of polymerization-based amplification.

Graphical abstract: Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

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Publication details

The article was received on 21 May 2012, accepted on 13 Aug 2012 and first published on 14 Aug 2012


Article type: Technical Innovation
DOI: 10.1039/C2LC40584A
Citation: Lab Chip, 2012,12, 4055-4058
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    Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

    K. Kaastrup and H. D. Sikes, Lab Chip, 2012, 12, 4055
    DOI: 10.1039/C2LC40584A

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