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Volume 159, 2012
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Precipitation of ACC in liposomes—a model for biomineralization in confined volumes

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Abstract

Biomineralizing organisms frequently precipitate minerals in small phospholipid bilayer-delineated compartments. We have established an in vitro model system to investigate the effect of confinement in attoliter to femtoliter volumes on the precipitation of calcium carbonate. In particular, we analyze the growth and stabilization of liposome-encapsulated amorphous calcium carbonate (ACC) nanoparticles using a combination of in situ techniques, cryo-transmission electron microscopy (Cryo-TEM), and small angle X-ray scattering (SAXS). Herein, we discuss ACC nanoparticle growth rate as a function of liposome size, carbon dioxide flux across the liposome membrane, pH, and osmotic pressure. Based on these experiments, we argue that the stabilization of ACC nanoparticles in liposomes is a consequence of a low nucleation rate (high activation barrier) of crystalline polymorphs of calcium carbonate.

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Publication details

The article was received on 30 Apr 2012, accepted on 06 Jun 2012, published on 07 Jun 2012 and first published online on 07 Jun 2012


Article type: Paper
DOI: 10.1039/C2FD20088K
Citation: Faraday Discuss., 2012,159, 345-356
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    Precipitation of ACC in liposomes—a model for biomineralization in confined volumes

    C. C. Tester, C. Wu, S. Weigand and D. Joester, Faraday Discuss., 2012, 159, 345
    DOI: 10.1039/C2FD20088K

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