Issue 3, 2012

A novel genetically encoded fluorescent protein as a Cu(i) indicator

Abstract

We constructed the copper(I)-binding domain of Mycobacterium tuberculosis (Mtb) CDC 1551 (residues 1–162) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP), and formed a novel genetically encoded fluorescent copper(I) responsive protein (PMtb). The sensitivity and selectivity to copper(I) of the PMtb was sought. The experiments showed that the copper(I)-binding domain of the PMtb was highly sensitive and selective towards copper(I).

Graphical abstract: A novel genetically encoded fluorescent protein as a Cu(i) indicator

Supplementary files

Additions and corrections

Article information

Article type
Communication
Submitted
29 May 2011
Accepted
07 Nov 2011
First published
29 Nov 2011

Dalton Trans., 2012,41, 727-729

A novel genetically encoded fluorescent protein as a Cu(I) indicator

X. Yan, X. Li, S. Lv and D. He, Dalton Trans., 2012, 41, 727 DOI: 10.1039/C1DT11002K

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