Issue 89, 2012
From the journal:

Chemical Communications

Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli†

Yanxiang Shi,a   Alejandro Buenoa  and  Wilfred A. van der Donk*a  

* Corresponding authors

a Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, 600 S Mathews Ave, Urbana, IL 61801, USA
E-mail: vddonk@uiuc.edu
Fax: +1 217 244 8533
Tel: +1 217 244 5360

Abstract

We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.

Graphical abstract: Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli

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Article information

DOI
https://doi.org/10.1039/C2CC36336D
Article type
Communication
Submitted
31 Aug 2012
Accepted
19 Sep 2012
First published
04 Oct 2012
This article is Open Access
Creative Commons BY license

Chem. Commun., 2012,48, 10966-10968

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Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli

Y. Shi, A. Bueno and W. A. van der Donk, Chem. Commun., 2012, 48, 10966 DOI: 10.1039/C2CC36336D

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