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Issue 33, 2012
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An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

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Abstract

O 6-Methyl-2′-deoxyguanosine (O6-Me-dG) is a mutagenic nucleotide in DNA. O6-Me-dG in DNA was rapidly and selectively modified by a functionality transfer reaction using the ODN incorporating 6-S-functionalized thioguanosine. Subsequent labelling of O6-Me-dG with the fluorescent FAM or biotin group via click chemistry has permitted the sensitive and selective detection of O6-Me-dG in DNA.

Graphical abstract: An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

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Publication details

The article was received on 06 Dec 2011, accepted on 01 Mar 2012 and first published on 01 Mar 2012


Article type: Communication
DOI: 10.1039/C2CC17621A
Citation: Chem. Commun., 2012,48, 3969-3971
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    An efficient and simple method for site-selective modification of O6-methyl-2′-deoxyguanosine in DNA

    K. Onizuka, T. Nishioka, Z. Li, D. Jitsuzaki, Y. Taniguchi and S. Sasaki, Chem. Commun., 2012, 48, 3969
    DOI: 10.1039/C2CC17621A

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