Issue 10, 2011
From the journal:

Chemical Science

Sensing coiled-coil proteins through conformational modulation of energy transfer processes – selective detection of the oncogenic transcription factor c-Jun†

Elena Pazos,a   Adrián Jiménez-Balsa,a   José L. Mascareñas*a  and  M. Eugenio Vázquez*a  

* Corresponding authors

a Departamento de Química Orgánica y Centro Singular de Investigación en Química Biológica y Materiales Moleculares, Unidad Asociada al CSIC, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
E-mail: eugenio.vazquez@usc.es, joseluis.mascarenas@usc.es.
Fax: (+34) 981 595 012
Tel: (+34) 881 815 738

Abstract

Designed terbium-chelating peptides were used to monitor the formation of leucine zipper associations. The sensing strategy is based on the modulation of the antenna effect from a Trp residue donor and the luminescence increase arising from the folding that takes place upon the formation of the coiled-coil structure. An application in the specific sensing of the oncogenic c-Jun transcription factor is demonstrated.

Graphical abstract: Sensing coiled-coil proteins through conformational modulation of energy transfer processes – selective detection of the oncogenic transcription factor c-Jun

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Article information

DOI
https://doi.org/10.1039/C1SC00108F
Article type
Edge Article
Submitted
23 Feb 2011
Accepted
05 Jul 2011
First published
21 Jul 2011

Chem. Sci., 2011,2, 1984-1987

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Sensing coiled-coil proteins through conformational modulation of energy transfer processes – selective detection of the oncogenic transcription factor c-Jun

E. Pazos, A. Jiménez-Balsa, J. L. Mascareñas and M. E. Vázquez, Chem. Sci., 2011, 2, 1984 DOI: 10.1039/C1SC00108F

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