Issue 1, 2011

PEGylation of surface protein filaments: coverage and impact on denaturation

Abstract

N-Hydroxysuccinimidyl functional polymers for multi-site bioconjugation to amine residues present in α-keratin protein filaments were synthesised via living radical polymerisation. Monomers containing di(ethylene glycol) and poly(ethylene glycol) were employed to give water soluble polymers which were characterised using 1H NMR and size exclusion chromatography (SEC). The denaturation temperature of the PEG–keratin conjugates was examined using differential scanning calorimetry (DSC) to show that after conjugation to damaged keratinous material, the structural properties of the protein were improved against thermal degradation. Scanning electron microscopy was used to visualise surface coverage.

Graphical abstract: PEGylation of surface protein filaments: coverage and impact on denaturation

Supplementary files

Article information

Article type
Paper
Submitted
10 May 2011
Accepted
13 Jun 2011
First published
18 Jul 2011

RSC Adv., 2011,1, 58-66

PEGylation of surface protein filaments: coverage and impact on denaturation

S. Slavin, E. Khoshdel and D. M. Haddleton, RSC Adv., 2011, 1, 58 DOI: 10.1039/C1RA00157D

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