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Issue 7, 2011
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Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

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Abstract

Facile immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) was achieved on electrospun nanofibres made of an alternating copolymer of styrene (Sty) and maleic anhydride (MAh). The immobilized enzymes were used to perform a cascade reaction oxidizing D-glucose by GOX to yield H2O2 and employing HRP to catalyze the reaction between the generated H2O2 and Ampliflu Red to yield Resorufin. The average activity of the immobilized HRP was determined to be 21% of that of the free enzyme.

Graphical abstract: Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

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Publication details

The article was received on 08 Feb 2011, accepted on 15 Mar 2011 and first published on 26 Mar 2011


Article type: Communication
DOI: 10.1039/C1PY00069A
Citation: Polym. Chem., 2011,2, 1479-1481
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    Facile immobilization of enzymes on electrospun poly(styrene-alt-maleic anhydride) nanofibres

    W. J. Cloete, C. Adriaanse, P. Swart and B. Klumperman, Polym. Chem., 2011, 2, 1479
    DOI: 10.1039/C1PY00069A

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