Issue 4, 2011
From the journal:

Molecular BioSystems

The RNA–DNA hybrid structure determined by EPR, CD and RNase H1†

Olga Romainczyk,a   Burkhard Endeward,b   Thomas F. Prisnerb  and  Joachim W. Engels*a  

* Corresponding authors

a Institute of Organic Chemistry and Chemical Biology, Max-von-Laue-Str. 7, 60438 Frankfurt am Main, Germany
E-mail: Joachim.engels@chemie.uni-frankfurt.de
Fax: +49 69-79829148
Tel: +49 69-79829150

b Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Max-von-Laue-Str. 7, 60438 Frankfurt am Main, Germany
E-mail: prisner@prisner.de

Abstract

A or B: RNA–DNA hybrids, key intermediates in gene regulation, were classified by pulsed electron–electron double resonance (PELDOR) in combination with CD spectroscopy into two classes, interpreted as A- and B-like structures. RNase H1 cleavage of these hybrids is in full agreement with these assignments, cleaving the hybrids with A-like geometry preferentially. This combined analytical approach allows the interpretation and eventually the design of more easily cleavable hybrids as needed for the antisense technology.

Graphical abstract: The RNA–DNA hybrid structure determined by EPR, CD and RNase H1

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0MB00258E
Article type
Communication
Submitted
04 Nov 2010
Accepted
07 Feb 2011
First published
19 Feb 2011
This article is Open Access

Mol. BioSyst., 2011,7, 1050-1052

Permissions

Request permissions

The RNA–DNA hybrid structure determined by EPR, CD and RNase H1

O. Romainczyk, B. Endeward, T. F. Prisner and J. W. Engels, Mol. BioSyst., 2011, 7, 1050 DOI: 10.1039/C0MB00258E

This is an Open Access article. The full version of this article can be posted on a website/blog, posted on an intranet, photocopied, emailed, distributed in a course pack or distributed in Continuing Medical Education (CME) materials provided that it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements