Issue 2, 2011
From the journal:

Molecular BioSystems

Time and space resolved uptake study of silicananoparticles by human cells†

Kayle Shapero,‡a   Federico Fenaroli,‡ab   Iseult Lynch,a   David C. Cottell,b   Anna Salvati*a  and  Kenneth A. Dawson*a  

* Corresponding authors

a Centre for BioNano Interactions, School of Chemistry and Chemical Biology, UCD Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
E-mail: Kenneth@fiachra.ucd.ie, Anna.Salvati@cbni.ucd.ie
Fax: +353 (0)1 716 2415
Tel: +353 (0)1 716 6928

b Electron Microscopy Laboratory, University College Dublin, Belfield, Dublin 4, Ireland

Abstract

A spatio-temporal mapping of the uptake of silica (SiO2) nanoparticles of different sizes by lung epithelial cells has been obtained. Based on high control of nanoparticle dispersion in cell media and cell exposure, one obtains reproducible and quantitative time-resolved data using a combination of flow cytometry, fluorescence and electron microscopies. We are thereby able to give a rather detailed account of the journey of SiO2 nanoparticles from the early events of uptake to their final sub-cellular localization.

Graphical abstract: Time and space resolved uptake study of silica nanoparticles by human cells

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Article information

DOI
https://doi.org/10.1039/C0MB00109K
Article type
Paper
Submitted
21 Jul 2010
Accepted
01 Sep 2010
First published
29 Sep 2010

Mol. BioSyst., 2011,7, 371-378

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Time and space resolved uptake study of silica nanoparticles by human cells

K. Shapero, F. Fenaroli, I. Lynch, D. C. Cottell, A. Salvati and K. A. Dawson, Mol. BioSyst., 2011, 7, 371 DOI: 10.1039/C0MB00109K

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