Microfluidic approaches for gene delivery and gene therapy

Jungkyu Kim; Inseong Hwang; Derek Britain; Taek Dong Chung; Yu Sun; Deok-Ho Kim

doi:10.1039/C1LC20766K

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Lab on a Chip

Issue 23, 2011

Miniaturisation for chemistry, physics, biology, materials science and bioengineering

Impact Factor 5.67 24 Issues per Year Indexed in MEDLINE

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Microfluidic approaches for gene delivery and gene therapy

Jungkyu Kim,^a Inseong Hwang,^b Derek Britain,^c Taek Dong Chung,^b Yu Sun*^d and Deok-Ho Kim*^c

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Department of Chemistry and Electrical Engineering, University of California, Berkeley, USA

Department of Chemistry, Seoul National University, Seoul, Korea

Department of Bioengineering, University of Washington, Seattle, USA
E-mail: deokho@uw.edu

Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Canada
E-mail: sun@mie.utoronto.ca

Lab Chip, 2011,11, 3941-3948

DOI: 10.1039/C1LC20766K
Received 16 Aug 2011, Accepted 10 Oct 2011
First published online 26 Oct 2011

This article is part of themed collection: Lab on a Chip 2011 Review Articles

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Abstract
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Recent advances in microfluidics have created new and exciting prospects for gene delivery and therapy. The micro-scaled environment within microfluidic systems enables precise control and optimization of multiple processes and techniques used in gene transfection and the production of gene and drug transporters. Traditional non-viral gene transfection methods, such as electroporation, microinjection and optical gene transfection, are improved from the use of innovative microfluidic systems. Additionally, microfluidic systems have also made the production of many viral and non-viral vectors controlled, automated, and reproducible. In summary, the development and application of microfluidic systems are producing increased efficiency in gene delivery and promise improved gene therapy results.

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Jungkyu Kim
Inseong Hwang
Derek Britain
Taek Dong Chung
Yu Sun
Deok-Ho Kim

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