Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 22, 2011
Previous Article Next Article

Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Author affiliations

Abstract

There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.

Graphical abstract: Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 24 May 2011, accepted on 08 Sep 2011 and first published on 27 Sep 2011


Article type: Method
DOI: 10.1039/C1LC20447E
Citation: Lab Chip, 2011,11, 3908-3914
  •   Request permissions

    Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

    J. C. Alexander, A. Pandit, G. Bao, D. Connolly and Y. Rochev, Lab Chip, 2011, 11, 3908
    DOI: 10.1039/C1LC20447E

Search articles by author