Issue 22, 2011

Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Abstract

There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.

Graphical abstract: Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Article information

Article type
Method
Submitted
24 May 2011
Accepted
08 Sep 2011
First published
27 Sep 2011

Lab Chip, 2011,11, 3908-3914

Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

J. C. Alexander, A. Pandit, G. Bao, D. Connolly and Y. Rochev, Lab Chip, 2011, 11, 3908 DOI: 10.1039/C1LC20447E

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