Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Jennifer Claire Alexander; Abhay Pandit; Gang Bao; David Connolly; Yury Rochev

doi:10.1039/C1LC20447E

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Lab on a Chip

Issue 22, 2011

Miniaturisation for chemistry, physics, biology, materials science and bioengineering

Impact Factor 5.67 24 Issues per Year Indexed in MEDLINE

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Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

Jennifer Claire Alexander,^a Abhay Pandit,^a Gang Bao,^b David Connolly^c and Yury Rochev*^a^c

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Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway, Ireland
E-mail: yury.rochev@nuigalway.ie ;
Fax: +353-9149-4596 ;
Tel: +353-9149-2806

Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, USA

National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland

Lab Chip, 2011,11, 3908-3914

DOI: 10.1039/C1LC20447E
Received 24 May 2011, Accepted 08 Sep 2011
First published online 27 Sep 2011

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There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.

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Jennifer Claire Alexander
Abhay Pandit
Gang Bao
David Connolly
Yury Rochev

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