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Issue 22, 2011
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Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

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Abstract

There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.

Graphical abstract: Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

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Publication details

The article was received on 24 May 2011, accepted on 08 Sep 2011 and first published on 27 Sep 2011


Article type: Method
DOI: 10.1039/C1LC20447E
Citation: Lab Chip, 2011,11, 3908-3914
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    Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons

    J. C. Alexander, A. Pandit, G. Bao, D. Connolly and Y. Rochev, Lab Chip, 2011, 11, 3908
    DOI: 10.1039/C1LC20447E

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