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Issue 14, 2011
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Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments

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Abstract

We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose–responses between the chip and the 96-well plate controls was found. The IC50 value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices.

Graphical abstract: Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments

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Publication details

The article was received on 21 Feb 2011, accepted on 21 Apr 2011 and first published on 01 Jun 2011


Article type: Paper
DOI: 10.1039/C1LC20147F
Citation: Lab Chip, 2011,11, 2333-2342
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    Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments

    L. Hsiung, C. Chiang, C. Wang, Y. Huang, C. Kuo, J. Cheng, C. Lin, V. Wu, H. Chou, D. Jong, H. Lee and A. M. Wo, Lab Chip, 2011, 11, 2333
    DOI: 10.1039/C1LC20147F

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