Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments

Lo-Chang Hsiung; Chi-Ling Chiang; Chen-Ho Wang; Yu-Hsu Huang; Ching-Te Kuo; Ji-Yen Cheng; Ching-Hung Lin; Victoria Wu; Hsien-Yeh Chou; De-Shien Jong; Hsinyu Lee; Andrew M. Wo

doi:10.1039/C1LC20147F

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Lab on a Chip

Issue 14, 2011

Miniaturisation for chemistry, physics, biology, materials science and bioengineering

Impact Factor 5.67 24 Issues per Year Indexed in MEDLINE

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Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments

Lo-Chang Hsiung,^a Chi-Ling Chiang,^b Chen-Ho Wang,^a Yu-Hsu Huang,^a Ching-Te Kuo,^a Ji-Yen Cheng,^c Ching-Hung Lin,^d^e Victoria Wu,^a Hsien-Yeh Chou,^a De-Shien Jong,^f Hsinyu Lee*^b and Andrew M. Wo*^a

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Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
E-mail: andrew@iam.ntu.edu.tw

Institute of Zoology, National Taiwan University, Taipei, Taiwan
E-mail: hsinyu@ ntu.edu.tw

Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan

Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan

College of Medicine, National Taiwan University, Taipei, Taiwan

Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan

Lab Chip, 2011,11, 2333-2342

DOI: 10.1039/C1LC20147F
Received 21 Feb 2011, Accepted 21 Apr 2011
First published online 01 Jun 2011

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We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose–responses between the chip and the 96-well plate controls was found. The IC₅₀ value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices.

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