Jump to main content
Jump to site search

Issue 34, 2011
Previous Article Next Article

Radio-frequency induced in vitro thermal ablation of cancer cells by EGF functionalized carbon-coated magnetic nanoparticles

Author affiliations

Abstract

Carbon-shelled, iron-based magnetic nanoparticles (C/Fe MNPs) were found to act as strong heat generating agents when exposed to radio-frequency (RF) energy with the ability to thermally destroy cancer cells. In order to efficiently deliver MNPs to cancer cells and to enhance the effectiveness of the RF treatment, human epidermal growth factor (EGF) was bioconjugated with the C/Fe MNPs for their specific delivery to two cancer cell lines, MCF-7 breast cancer cells and Panc-1 pancreatic cancer cells, respectively. These cell lines overexpress the epidermal growth factor receptors (EGFRs) and were used in this study as models. EGF-MNPs have shown higher surface binding efficiency towards the MCF-7 cells based on the comparative ζ-potential measurements. Confocal optical microscopy further confirmed that EGF-bioconjugated MNPs highly accumulated around and inside of these cancer cells. RF treatment was found to destroy 92.8% of MCF-7 breast cancer cells during 10 minutes of treatment when EGF was bound to the nanoparticles, while 37.3% of cells died when MNPs alone were used in identical conditions. Panc-1 cancer cells exhibit a higher resistance than MCF-7 cells when they were exposed to MNPs or RF treatment. Cytotoxicity studies demonstrated that the EGF-C/Fe MNP bioconjugates present lower toxicity compared to the C/Fe MNP. Caspase assay studies demonstrated that the MCF-7 cancer cells underwent an apoptotic process by the caspase 3 deficiency pathway showing no evidence of morphological changes such as membrane blebbing.

Graphical abstract: Radio-frequency induced in vitro thermal ablation of cancer cells by EGF functionalized carbon-coated magnetic nanoparticles

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 07 Feb 2011, accepted on 17 Jun 2011 and first published on 27 Jul 2011


Article type: Paper
DOI: 10.1039/C1JM10569H
Citation: J. Mater. Chem., 2011,21, 12761-12769
  •   Request permissions

    Radio-frequency induced in vitro thermal ablation of cancer cells by EGF functionalized carbon-coated magnetic nanoparticles

    A. Karmakar, Y. Xu, M. W. Mahmood, Y. Zhang, L. M. Saeed, T. Mustafa, S. Ali, A. R. Biris and A. S. Biris, J. Mater. Chem., 2011, 21, 12761
    DOI: 10.1039/C1JM10569H

Search articles by author