Jump to main content
Jump to site search

Issue 12, 2011
Previous Article Next Article

Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance

Author affiliations

Abstract

Biomass is one of the most abundant potential sustainable sources for fuel and material production, however to fully realize this potential an improved understanding of lignocellulosic recalcitrance must be developed. In an effort to appreciate the underlying phenotypic, biochemical and morphological properties associated with the reduced recalcitrance observed in tension stress-induced reaction wood, we report the increased enzymatic sugar yield and corresponding chemical and ultrastructural properties of Populus tension wood. Populus tremula x alba (PTA) was grown under tension and stem segments containing three different wood types: normal wood (NW), tension wood (TW) from the elongated stem side and opposite wood (OW) from the compressed stem side were collected. A variety of analytical techniques were used to describe changes occurring as a result of the tension stress-induced formation of a gelatinous cell wall layer (G-layer). For example, gel permeation chromatography (GPC) and 13C solid-state nuclear magnetic resonance (NMR) revealed that the molecular weight and crystallinity of cellulose in TW is greater than that of cellulose acquired from NW. Whole cell ionic liquid and other solid-state NMR analysis detailed the structure of lignin and hemicellulose in the samples, detecting the presence of variations in lignin and hemicellulose sub-units, linkages and semi-quantitatively estimating the relative amounts of syringyl (S), guaiacyl (G) and p-hydroxybenzoate (PB) monolignol units. It was confirmed that TW displayed an increase in PB or H-like lignin and S to G ratio from 1.25 to 1.50 when compared to the NW sample. Scanning electron microscopy (SEM) and coherent anti-Stokes Raman scattering (CARS) were also used to evaluate the morphology and corresponding spatial distribution of the major lignocellulosic components. We found changes in a combination of cell wall properties appear to influence recalcitrance more than any single factor alone.

Graphical abstract: Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 04 Jul 2011, accepted on 02 Sep 2011 and first published on 04 Oct 2011


Article type: Paper
DOI: 10.1039/C1EE02073K
Citation: Energy Environ. Sci., 2011,4, 4962-4971
  •   Request permissions

    Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance

    M. Foston, C. A. Hubbell, R. Samuel, S. Jung, H. Fan, S. Ding, Y. Zeng, S. Jawdy, M. Davis, R. Sykes, E. Gjersing, G. A. Tuskan, U. Kalluri and A. J. Ragauskas, Energy Environ. Sci., 2011, 4, 4962
    DOI: 10.1039/C1EE02073K

Search articles by author