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Issue 6, 2011
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2D IR photon echo of azido-probes for biomolecular dynamics

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Abstract

The vibrations in the azido-, N3, asymmetric stretching region of 2′-azido-2′-deoxyuridine (N3dU) are examined by two-dimensional infrared spectroscopy. In water and tetrahydrofuran (THF), the spectra display a single sharp diagonal peak that shows solvent sensitivity. The frequency–frequency correlation time in water is 1.5 ps, consistent with H-bond making and breaking dynamics. The 2D IR spectrum is reproduced for N3dU in water based on a model correlation function and known linear response functions. Its large extinction coefficient, vibrational frequency outside the protein and nucleic acid IR absorption, and sensitivity to water dynamics render –N3 a very useful probe for 2D IR and other nonlinear IR studies: its signal is ca. 100 times that of nitriles.

Graphical abstract: 2D IR photon echo of azido-probes for biomolecular dynamics

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Publication details

The article was received on 27 Aug 2010, accepted on 03 Nov 2010 and first published on 02 Dec 2010


Article type: Paper
DOI: 10.1039/C0CP01625J
Citation: Phys. Chem. Chem. Phys., 2011,13, 2237-2241
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    2D IR photon echo of azido-probes for biomolecular dynamics

    M. J. Tucker, X. S. Gai, E. E. Fenlon, S. H. Brewer and R. M. Hochstrasser, Phys. Chem. Chem. Phys., 2011, 13, 2237
    DOI: 10.1039/C0CP01625J

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