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San José State University, Department of Chemistry, One Washington Square, San José, USA
E-mail: lionel.cheruzel@sjsu.edu
; Fax: +1 408 924 4945
; Tel: +1 408 924 5283
Chem. Commun., 2011,47, 11936-11938
DOI:
10.1039/C1CC15124J
Received
17 Aug 2011,
Accepted
15 Sep 2011
First published online
05 Oct 2011
We have developed hybrid P450BM3 enzymes consisting of a Ru(II)-diimine photosensitizer covalently attached to non-native single cysteine residues of P450 BM3heme domain mutants. These enzymes are capable, upon light activation, of selectively hydroxylating lauric acid with 40 times higher total turnover numbers compared to the peroxide shunt.
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