Issue 37, 2011
From the journal:

Chemical Communications

Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays†

Yasuhiko Iwasaki*a  and  Tatsuya Otaa  

* Corresponding authors

a Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka 564-8680, Japan
E-mail: yasu.bmt@kansai-u.ac.jp
Fax: +81-6-6368-0090
Tel: +81-6-6368-0090

Abstract

In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol–ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG410K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.

Graphical abstract: Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

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Article information

DOI
https://doi.org/10.1039/C1CC12948A
Article type
Communication
Submitted
19 May 2011
Accepted
02 Aug 2011
First published
19 Aug 2011

Chem. Commun., 2011,47, 10329-10331

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Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays

Y. Iwasaki and T. Ota, Chem. Commun., 2011, 47, 10329 DOI: 10.1039/C1CC12948A

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