Issue 18, 2011
From the journal:

Chemical Communications

Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation†

Armin Thalhammer,a   Anders S. Hansen,‡a   Afaf H. El-Sagheer,bc   Tom Brownb  and  Christopher J. Schofield*a  

* Corresponding authors

a Department of Chemistry and the Oxford Centre for Integrative Systems Biology, Chemistry Research Laboratory, Oxford, UK
E-mail: Christopher.schofield@chem.ox.ac.uk
Fax: +44 (0)1865 285002
Tel: +44 (0)1865 275625

b School of Chemistry, University of Southampton, Southampton, UK

c Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez Canal University, Suez, Egypt

Abstract

Cytosine-5-methylation stabilises DNA duplexes and is associated with transcriptional repression; 5-methylcytosine undergoes hydroxylation to 5-hydroxymethylcytosine, a modification of unknown biological function. Spectroscopic and calorimetric analyses show that 5-hydroxymethylcytosine introduction reverses the stabilising effect of 5-methylcytosine, suggesting that in some contexts, 5-methylcytosine hydroxylation may, along with other factors, contribute to the alleviation of transcriptional repression.

Graphical abstract: Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0CC05671E
Article type
Communication
Submitted
20 Dec 2010
Accepted
16 Mar 2011
First published
31 Mar 2011

Chem. Commun., 2011,47, 5325-5327

Permissions

Request permissions

Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

A. Thalhammer, A. S. Hansen, A. H. El-Sagheer, T. Brown and C. J. Schofield, Chem. Commun., 2011, 47, 5325 DOI: 10.1039/C0CC05671E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements