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Issue 22, 2011
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High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

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Abstract

A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.

Graphical abstract: High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

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Publication details

The article was received on 01 Aug 2011, accepted on 14 Sep 2011 and first published on 29 Sep 2011


Article type: Communication
DOI: 10.1039/C1AN15684E
Citation: Analyst, 2011,136, 4670-4673
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    High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection

    A. Knežević, J. Bones, S. K. Kračun, O. Gornik, P. M. Rudd and G. Lauc, Analyst, 2011, 136, 4670
    DOI: 10.1039/C1AN15684E

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