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Issue 15, 2011
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A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

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Abstract

Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg6 due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg6 with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL−1 can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg6 are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin.

Graphical abstract: A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

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Publication details

The article was received on 20 Mar 2011, accepted on 20 May 2011 and first published on 22 Jun 2011


Article type: Paper
DOI: 10.1039/C1AN15224F
Citation: Analyst, 2011,136, 3136-3141
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    A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles

    W. Xue, G. Zhang and D. Zhang, Analyst, 2011, 136, 3136
    DOI: 10.1039/C1AN15224F

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