Issue 4, 2011
From the journal:

Analyst

Combing DNAzyme with single-walled carbon nanotubes for detection of Pb(ii) in water†

Jingjing Yao,a   Jishan Li,ab   Jeremy Owensc  and  Wenwan Zhong*a  

* Corresponding authors

a Department of Chemistry, University of California, Riverside, Riverside, CA, USA
E-mail: wenwan.zhong@ucr.edu
Fax: +1-951-827-4713
Tel: +1-951-827-4925

b College of Chemistry and Chemical Engineering, State Key Laboratory for Chemo/Biosensing and Chemometrics, Hunan University, Changsha, P.R. China

c Department of Earth Sciences, University of California, Riverside, Riverside, CA, USA

Abstract

A sensitive and simple assay for the detection of Pb2+ in aqueous solutions is reported. It takes advantage of the high affinity between single-stranded DNA (ssDNA) and single-walled carbon nanotubes (SWCNT) as well as the capability of SWCNT in fluorescence quenching. Lead(II) catalyzes the cleavage of a fluorescently labeled DNA substrate by a DNAzyme, which releases the single-stranded product to be adsorbed onto a SWCNT. The decrease in fluorescence is proportional to the Pb2+ concentration. Concentrations as low as 1 nM Pb2+ in water could be detected and the detection range spans over 5 orders of magnitude. The unique combination of Pb-specific DNAzyme with SWCNT produces a universal, facile and cost-effective sensing platform for lead ions. The concept can be applied to the design of detection assays for other metal ions or small molecules.

Graphical abstract: Combing DNAzyme with single-walled carbon nanotubes for detection of Pb(ii) in water

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Article information

DOI
https://doi.org/10.1039/C0AN00709A
Article type
Paper
Submitted
11 Sep 2010
Accepted
08 Nov 2010
First published
13 Dec 2010

Analyst, 2011,136, 764-768

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Combing DNAzyme with single-walled carbon nanotubes for detection of Pb(II) in water

J. Yao, J. Li, J. Owens and W. Zhong, Analyst, 2011, 136, 764 DOI: 10.1039/C0AN00709A

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