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Issue 18, 2011
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Direct observation of single flexible polymers using single stranded DNA

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Abstract

Over the last 15 years, double stranded DNA (dsDNA) has been used as a model polymeric system for nearly all single polymer dynamics studies. However, dsDNA is a semiflexible polymer with markedly different molecular properties compared to flexible chains, including synthetic organic polymers. In this work, we report a new system for single polymer studies of flexible chains based on single stranded DNA (ssDNA). We developed a method to synthesize ssDNA for fluorescence microscopy based on rolling circle replication, which generates long strands (>65 kb) of ssDNA containing “designer” sequences, thereby preventing intramolecular base pair interactions. Polymers are synthesized to contain amine-modified bases randomly distributed along the backbone, which enables uniform labelling of polymer chains with a fluorescent dye to facilitate fluorescence microscopy and imaging. Using this approach, we synthesized ssDNA chains with long contour lengths (>30 μm) and relatively low dye loading ratios (∼1 dye per 100 bases). In addition, we used epifluorescence microscopy to image single ssDNA polymer molecules stretching in flow in a microfluidic device. Overall, we anticipate that ssDNA will serve as a useful model system to probe the dynamics of polymeric materials at the molecular level.

Graphical abstract: Direct observation of single flexible polymers using single stranded DNA

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Publication details

The article was received on 19 Feb 2011, accepted on 03 May 2011 and first published on 02 Jun 2011


Article type: Paper
DOI: 10.1039/C1SM05297G
Citation: Soft Matter, 2011,7, 8005-8012
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    Direct observation of single flexible polymers using single stranded DNA

    C. Brockman, S. J. Kim and C. M. Schroeder, Soft Matter, 2011, 7, 8005
    DOI: 10.1039/C1SM05297G

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