Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 7, 2011
Previous Article Next Article

The binding of polyvalent galactosides to the lectin Ricinus communis agglutinin 120 (RCA120): an ITC and SPR study

Author affiliations

Abstract

Mono- and polyvalent galactosides have been investigated with respect to their binding to the plant lectin Ricinus communis agglutinin 120 (RCA120). Thermodynamic parameters (Ka, ΔG, ΔH, ΔS and n) have been determined by isothermal titration calorimetry (ITC) and kinetics of binding (ka and kd) measured by surface plasmon resonance (SPR). ITC analysis using a single set of sites model found a non-statistical increase in avidity with increasing valency with the largest ligand displaying a greater than 20-fold increase in Ka compared to its monomeric precursor after correction for valency; binding was found to be enthalpically driven. SPR analysis supports the avidity increase but values of Ka observed were up to 100-fold greater than those measured by ITC. The large discrepancy between the two measurements is rationalized by the polyvalent–polyvalent interaction that is measured by SPR.

Graphical abstract: The binding of polyvalent galactosides to the lectin Ricinus communis agglutinin 120 (RCA120): an ITC and SPR study

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 19 Jan 2011, accepted on 10 Mar 2011 and first published on 04 Apr 2011


Article type: Paper
DOI: 10.1039/C1PY00030F
Citation: Polym. Chem., 2011,2, 1552-1560
  •   Request permissions

    The binding of polyvalent galactosides to the lectin Ricinus communis agglutinin 120 (RCA120): an ITC and SPR study

    S. G. Spain and N. R. Cameron, Polym. Chem., 2011, 2, 1552
    DOI: 10.1039/C1PY00030F

Search articles by author