Issue 7, 2011
From the journal:

Polymer Chemistry

Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion†

Saadyah E. Averick,a   Andrew J. D. Magenau,a   Antonina Simakova,a   Bradley F. Woodman,b   Andrew Seong,a   Ryan A. Mehlb  and  Krzysztof Matyjaszewski*a  

* Corresponding authors

a Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA, USA
E-mail: km3b@andrew.cmu.edu
Fax: +1 412-268-6897
Tel: +1 412-268-3209

b Departments of Chemistry, Franklin & Marshall College, Lancaster, PA, USA
E-mail: ryan.mehl@fandm.edu
Fax: +1 717-291-4343
Tel: +1 717-291-4125

Abstract

Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein–nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.

Graphical abstract: Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion

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Article information

DOI
https://doi.org/10.1039/C1PY00050K
Article type
Communication
Submitted
29 Jan 2011
Accepted
07 Mar 2011
First published
24 Mar 2011

Polym. Chem., 2011,2, 1476-1478

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Covalently incorporated protein–nanogels using AGET ATRP in an inverse miniemulsion

S. E. Averick, A. J. D. Magenau, A. Simakova, B. F. Woodman, A. Seong, R. A. Mehl and K. Matyjaszewski, Polym. Chem., 2011, 2, 1476 DOI: 10.1039/C1PY00050K

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