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Issue 9, 2010
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Rapid thermal lysis of cells using silicondiamond microcantilever heaters

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Abstract

This paper presents the design and application of microcantilever heaters for biochemical applications. Thermal lysis of biological cells was demonstrated as a specific example. The microcantilever heaters, fabricated from selectively doped single crystal silicon, provide local resistive heating with highly uniform temperature distribution across the cantilevers. Very importantly, the microcantilever heaters were coated with a layer of 100 nm thick electrically insulating ultrananocrystalline diamond (UNCD) layer used for cell immobilization on the cantilever surface. Fibroblast cells or bacterial cells were immobilized on the UNCD/cantilever surfaces and thermal lysis was demonstrated via optical fluorescence microscopy. Upon electrical heating of the cantilever structures to 93 °C for 30 seconds, fibroblast cell and nuclear membrane were compromised and the cells were lysed. Over 90% of viable bacteria were also lysed after 15 seconds of heating at 93 °C. This work demonstrates the utility of siliconUNCD heated microcantilevers for rapid cell lysis and forms the basis for other rapid and localized temperature-regulated microbiological experiments in cantilever-based lab on chip applications.

Graphical abstract: Rapid thermal lysis of cells using silicon–diamond microcantilever heaters

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Publication details

The article was received on 11 Nov 2009, accepted on 25 Jan 2010 and first published on 12 Mar 2010


Article type: Paper
DOI: 10.1039/B923791G
Citation: Lab Chip, 2010,10, 1135-1141
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    Rapid thermal lysis of cells using silicondiamond microcantilever heaters

    N. Privorotskaya, Y. Liu, J. Lee, H. Zeng, J. A. Carlisle, A. Radadia, L. Millet, R. Bashir and W. P. King, Lab Chip, 2010, 10, 1135
    DOI: 10.1039/B923791G

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