Jump to main content
Jump to site search

Issue 24, 2010
Previous Article Next Article

Core/shell nanoparticles as hybrid platforms for the fabrication of a hydrogen peroxide biosensor

Author affiliations

Abstract

Core/shell nanoparticles consisting of a Fe3O4 nanoparticle core and a mesoporous silica shell (Fe3O4/m-silica) were used as a matrix for immobilization of horseradish peroxidase (HRP) enzyme and subsequent design of an amperometric hydrogen peroxide biosensor. HRP enzyme was immobilized on the core/shell nanoparticles through the electrostatic interaction between oppositely charged HRP enzyme and the silica shell at neutral pH. The enzyme–core/shell nanoparticle hybrid material was deposited on screen printed electrodes and further characterized by ultraviolet–visible (UV-vis) spectroscopy and scanning electron microscopy (SEM). This set up was used as a biosensor to detect hydrogen peroxide. The hydrogen peroxide biosensor showed a detection limit of 4.3 × 10−7 M, at a signal-to-noise ratio of 3, and a sensitivity of 84.4 μA mM−1 cm2.

Graphical abstract: Core/shell nanoparticles as hybrid platforms for the fabrication of a hydrogen peroxide biosensor

Back to tab navigation

Publication details

The article was received on 27 Jan 2010, accepted on 25 Mar 2010 and first published on 14 May 2010


Article type: Paper
DOI: 10.1039/C0JM00182A
Citation: J. Mater. Chem., 2010,20, 5030-5034
  •   Request permissions

    Core/shell nanoparticles as hybrid platforms for the fabrication of a hydrogen peroxide biosensor

    Y. Won, D. Aboagye, H. S. Jang, A. Jitianu and L. A. Stanciu, J. Mater. Chem., 2010, 20, 5030
    DOI: 10.1039/C0JM00182A

Search articles by author

Spotlight

Advertisements