Issue 36, 2010
From the journal:

Dalton Transactions

Synthesis of hybrid transition-metalloproteinsviathiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes†

René den Heeten,a   Bianca K. Muñoz,b   Gina Popa,b   Wouter Laan*b  and  Paul C. J. Kamer*b  

* Corresponding authors

a Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 166, Amsterdam, The Netherlands

b EaStCHEM, Department of Chemistry, University of St. Andrews, St. Andrews, Fife, United Kingdom
E-mail: pcjk@st-andrews.ac.uk, wwl1@st-andrews.ac.uk
Fax: +44-1334-463808

Abstract

The preparation of hybrid transition metalloproteins by thiol-selective incorporation of organometallic rhodium- and ruthenium complexes is described. Phosphine ligands and two rhodium-diphosphine complexes bearing a carboxylic acid group were coupled to the cysteine of PYP R52G, yielding a metalloenzyme active in the rhodium catalyzed hydrogenation of dimethyl itaconate. The successful coupling was shown by 31P NMR spectroscopy and ESI mass spectroscopy. In addition wild-type PYP (PYP WT), PYP R52G and ALBP were successfully modified with a (η6-arene) ruthenium(II) phenanthroline complex via a maleimide linker.

Graphical abstract: Synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

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Article information

DOI
https://doi.org/10.1039/C0DT00239A
Article type
Paper
Submitted
31 Mar 2010
Accepted
10 Jun 2010
First published
06 Jul 2010

Dalton Trans., 2010,39, 8477-8483

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Synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

R. den Heeten, B. K. Muñoz, G. Popa, W. Laan and P. C. J. Kamer, Dalton Trans., 2010, 39, 8477 DOI: 10.1039/C0DT00239A

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