Issue 17, 2010
From the journal:

Chemical Communications

Detection of hetero-proteins–mesoporous silicaassembly by BRET†

Shun-ichi Matsuura,*a   Tatsuo Tsunoda,a   Toru Shiomi,a   Kengo Sakaguchi,b   Takaaki Hanaokaa  and  Fujio Mizukamia  

* Corresponding authors

a Research Center for Compact Chemical Process, National Institute of Advanced Industrial Science and Technology (AIST), Nigatake 4-2-1, Miyagino-ku, Sendai, Japan
E-mail: matsuura-shunichi@aist.go.jp
Fax: +81-22-237-3073
Tel: +81-22-237-3039

b Department of Applied Biological Science, Tokyo University of Science, Yamazaki 2641, Noda, Chiba, Japan

Abstract

The assembly of a hetero-protein (Renilla reniformis luciferase (Rluc) and a green fluorescence protein (sGFP)) encapsulated in folded-sheet mesoporous material with 7.1 nm pore diameter (FSM7.1), which was used for studying protein–protein interactions in pores of mesoporous silica, has been confirmed by the detection of bioluminescence resonance energy transfers (BRET).

Graphical abstract: Detection of hetero-proteins–mesoporous silica assembly by BRET

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Article information

DOI
https://doi.org/10.1039/B925607E
Article type
Communication
Submitted
08 Dec 2009
Accepted
23 Feb 2010
First published
09 Mar 2010

Chem. Commun., 2010,46, 2941-2943

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Detection of hetero-proteins–mesoporous silica assembly by BRET

S. Matsuura, T. Tsunoda, T. Shiomi, K. Sakaguchi, T. Hanaoka and F. Mizukami, Chem. Commun., 2010, 46, 2941 DOI: 10.1039/B925607E

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