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Issue 8, 2009
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Controlling the selection stringency of phage display using a microfluidic device

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Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

Graphical abstract: Controlling the selection stringency of phage display using a microfluidic device

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Publication details

The article was received on 24 Nov 2008, accepted on 18 Feb 2009 and first published on 03 Mar 2009


Article type: Communication
DOI: 10.1039/B820985E
Citation: Lab Chip, 2009,9, 1033-1036
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    Controlling the selection stringency of phage display using a microfluidic device

    Y. Liu, J. D. Adams, K. Turner, F. V. Cochran, S. S. Gambhir and H. T. Soh, Lab Chip, 2009, 9, 1033
    DOI: 10.1039/B820985E

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