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Issue 6, 2009
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Real-time monitoring of two-photon photopolymerization for use in fabrication of microfluidic devices

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Abstract

We report an improved method for production of microfluidic device masters using two-photon photopolymerization of SU-8 negative photoresist, which relies on a two-photon microscope (TPM) commonly used in imaging of biological samples. The device masters serve as negative relief structures for polydimethylsiloxane-based microfluidic devices. We observed that not only did the two-photon excitation of the SU-8 photoresist initiate crosslinking of the material in the region of the focus of the near-infrared laser beam (as expected) but it also resulted in emission of fluorescence in the visible range. The detected emission of SU-8 photoresist undergoing two-photon excitation displayed a strong correlation with the size of the polymerized objects produced during the exposure; this allowed the progress of the microfluidic master production process to be monitored in real-time. We demonstrate the use of the fluorescence detection during two-photon photopolymerization in the production of microfluidic devices, which were designed to trap individual yeast cells to be imaged with the same TPM used for microfluidic master writing.

Graphical abstract: Real-time monitoring of two-photon photopolymerization for use in fabrication of microfluidic devices

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Publication details

The article was received on 29 Sep 2008, accepted on 10 Nov 2008 and first published on 04 Dec 2008


Article type: Paper
DOI: 10.1039/B816993D
Citation: Lab Chip, 2009,9, 819-827
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    Real-time monitoring of two-photon photopolymerization for use in fabrication of microfluidic devices

    M. Stoneman, M. Fox, C. Zeng and V. Raicu, Lab Chip, 2009, 9, 819
    DOI: 10.1039/B816993D

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