Novel fluorescence enhancement IgE assay using a DNA aptamer

Abstract

In this paper, we demonstrate a fluorescence immunoglobulin E (IgE) assay probe based on a DNA aptamer. A Texas red-labeled short DNA strand (T-DNA) complementary with part of the IgE aptamer sequence was used to produce the fluorescence enhancement effected upon the binding of IgE to the aptamer. Another short DNA strand labeled with dabcyl quencher (Q-DNA) complementary with part of the aptamer sequence nearby the T-DNA location was used to lower the background fluorescence. The IgE can be detected in the concentration range from 9.2 × 10⁻¹¹ to 3.7 × 10⁻⁸ mol L⁻¹ with a detection limit of 5.7 × 10⁻¹¹ mol L⁻¹.