Issue 11, 2006
From the journal:

Chemical Communications

Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone†

Chunxia Zhao,a   Qing Dai,a   Takehiro Seino,a   Ying-Yu Cui,a   Seiichi Nishizawaab  and  Norio Teramae*ab  

* Corresponding authors

a Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan
E-mail: tera@anal.chem.tohoku.ac.jp
Fax: +81 22 7956552
Tel: +81 22 7956549

b CREST, Japan Science and Technology Agency (JST), Aoba-ku, Sendai 980-8578, Japan

Abstract

Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.

Graphical abstract: Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone

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Article information

DOI
https://doi.org/10.1039/B516575J
Article type
Communication
Submitted
22 Nov 2005
Accepted
12 Jan 2006
First published
30 Jan 2006

Chem. Commun., 2006, 1185-1187

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Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone

C. Zhao, Q. Dai, T. Seino, Y. Cui, S. Nishizawa and N. Teramae, Chem. Commun., 2006, 1185 DOI: 10.1039/B516575J

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