The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion

Marcus G. Friedel; Olivier Berteau; J. Carsten Pieck; Mohamed Atta; Sandrine Ollagnier-de-Choudens; Marc Fontecave; Thomas Carell

doi:10.1039/B514103F

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The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion†

Marcus G. Friedel,^a Olivier Berteau,^b J. Carsten Pieck,^a Mohamed Atta,^b Sandrine Ollagnier-de-Choudens,^b Marc Fontecave*^b and Thomas Carell*^a

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* Corresponding authors

^a Department of Chemistry and Biochemistry, LMU Munich, Butenandtstr. 5-13, Munich, Germany
E-mail: Thomas.Carell@cup.uni-muenchen.de
Fax: +49 89 2180 77756
Tel: +49 89 2180 77750

^b Laboratoire de Chimie et Biochemie des Centre Rédox Biologiques, UMR CEA-CNRS-Université Joseph Fourier n°. 5047, CEA Grenoble, DRDC-CB, 17 Avenue des Martyrs, Grenoble Cedex 9, France
E-mail: mfontecave@cea.fr
Fax: +33 4 3878 9124
Tel: +33 4 3878 9102

Abstract

The spore photoproduct lyase is a Fe–S/AdoMet DNA repair enzyme, which directly repairs spore lesions, induced by UV irradiation of spores, using an unknown radical mechanism. The air sensitive radical SAM enzyme was for the first time challenged with synthetically pure substrates. It was found that the enzyme recognizes a synthetic 5S-configured spore lesion without the central phosphodiester bond. The 5R-configured lesion is in contrast to current belief not a substrate.

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Supplementary files

Synthesis of the SP-isomers 8a and 8b, HPLC chromatograms of the separated isomers 7a and 7b, ¹H-NMR spectra from 8a and 8b, analytical data of the bridged SP-isomers 9a and 9b and NOE data for the isomers 8a/b and 9a/b PDF (615K)

Article information

DOI: https://doi.org/10.1039/B514103F
Article type: Communication
Submitted: 06 Oct 2005
Accepted: 10 Nov 2005
First published: 09 Dec 2005

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Chem. Commun., 2006, 445-447

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The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion

M. G. Friedel, O. Berteau, J. C. Pieck, M. Atta, S. Ollagnier-de-Choudens, M. Fontecave and T. Carell, Chem. Commun., 2006, 445 DOI: 10.1039/B514103F

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Chemical Communications

The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion†

Abstract

Supplementary files

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The spore photoproduct lyase repairs the 5S- and not the 5R-configured spore photoproduct DNA lesion

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