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Issue 8, 2006
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Sensitive and rapid electrochemical bioassay of glycosidase activity

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Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Graphical abstract: Sensitive and rapid electrochemical bioassay of glycosidase activity

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Publication details

The article was received on 26 Apr 2006, accepted on 27 Jun 2006 and first published on 06 Jul 2006


Article type: Communication
DOI: 10.1039/B605943K
Citation: Analyst, 2006,131, 889-891
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    Sensitive and rapid electrochemical bioassay of glycosidase activity

    T. Tangkuaram, J. Q. Gerlach, Y. Xiang, A. Kawde, Z. Dai, V. P. Bhavanandan, J. T. La Belle, W. Veerasai, L. Joshi and J. Wang, Analyst, 2006, 131, 889
    DOI: 10.1039/B605943K

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