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Issue 1, 2005
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Design and fabrication of an integrated cell processor for single embryo cell manipulation

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Abstract

This paper presents an integrated cell processor for the automatic handling of individual embryo cells. The integrated processor can perform various functions such as cell transport, isolation, orientation, and immobilization. These functions are indispensable and frequently used for the manipulation of single cells, but can only be carried out by a skillful operator. The purpose of this study was the integration and automation of these functions for effective cell manipulation, using a MEMS approach. The isolation of a cell was performed using polypyrrole (PPy) valves in a microchannel into which cells were transported. The orientation of cells was controlled by electrorotation (ER), and the target cell was immobilized by suction from a microhole. All of these functions were seamlessly realized on a single chip. Excellent experimental results with mouse (B6CBA) embryo cells showed that this device could substitute for routine and cumbersome manual work. It is expected that the integrated chip will contribute significantly to faster and more reliable manipulation of cells.

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Publication details

The article was received on 07 May 2004, accepted on 28 Jun 2004 and first published on 07 Oct 2004


Article type: Paper
DOI: 10.1039/B404990J
Citation: Lab Chip, 2005,5, 91-96
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    Design and fabrication of an integrated cell processor for single embryo cell manipulation

    J. Park, S. Jung, Y. Kim, B. Kim, S. Lee and J. Park, Lab Chip, 2005, 5, 91
    DOI: 10.1039/B404990J

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