Issue 24, 2004
From the journal:

Organic & Biomolecular Chemistry

NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly†

Masato Oikawa,a   Tetsuya Shintaku,a   Naohiro Fukuda,a   Harald Sekljic,a   Yoshiyuki Fukase,a   Hiroaki Yoshizaki,a   Koichi Fukase*a  and  Shoichi Kusumotoa  

* Corresponding authors

a Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, Japan
E-mail: koichi@chem.sci.osaka-u.ac.jp

Abstract

The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules.

Graphical abstract: NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly

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Article information

DOI
https://doi.org/10.1039/B410544C
Article type
Paper
Submitted
12 Jul 2004
Accepted
30 Sep 2004
First published
08 Nov 2004

Org. Biomol. Chem., 2004,2, 3557-3565

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NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly

M. Oikawa, T. Shintaku, N. Fukuda, H. Sekljic, Y. Fukase, H. Yoshizaki, K. Fukase and S. Kusumoto, Org. Biomol. Chem., 2004, 2, 3557 DOI: 10.1039/B410544C

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