A photoelectric method for analyzing cell-free apoptosis induced by dATP

Abstract

A new photoelectric method for analyzing cell-free apoptosis had been developed with ITO (a transparent electrode of indium–tin oxide coated borosilicate) technology. The C5710 mice liver nuclei responded with a negative photoelectric current pulse to white light (200–800nm). During the apoptosis of liver nuclei induced by dATP (deoxyadenosine-5-triphosphate), the photoelectric current showed a dynamic decrease, which was in accordance with the results of fluorescence microscopy and agarose gel electrophoresis. This photoelectric analytical method might provide a rapid and sensitive way to evaluate apoptosis quickly and in a continuous fashion.