Biosynthesis of vitamins B1 and B6 in Escherichia coli: concurrent incorporation of 1-deoxy-D-xylulose into thiamin (B1) and pyridoxol (B6)

Abstract

It is shown by ¹³C NMR spectroscopy that, in Escherichia coli mutant WG2, the C-2,-3 bond of [2,3-¹³C₂]-1-deoxy-D-xylulose 2 enters C-4,-5 of the thiazole unit of thiamin (B₁) 1 and C-2,-3 of pyridoxol (B₆) 3, providing the first direct evidence that the intact C₅ chain of 1-deoxy-D-xylulose is incorporated concurrently into each of the two B Vitamins.