Issue 15, 1995

Reactivity of [ RuIII(edta)(H2O)] with nucleic bases, nucleosides and DNA (calf-thymus) in aqueous solution (etda = ethylenediamine-N,N,N′,N′-tetraacetate)

Abstract

Reactions of [RuIII(edta)(H2O)](edta = ethylenediamine-N,N,N′,N′-tetraacetate) with L′(L′= adenine, adenosine, cytosine, cytidine or thymine) and calf-thymus DNA were studied by spectrophotometric, electrochemical and kinetic methods. Spectral features of the substituted product [RuIII(edta)L′] complexes were characterised by a strong ligand-to-metal charge transfer (l.m.c.t.) band in the UV region (293–309 nm). The E½ values for the RuIII–RuII couple for [RuIII(edta)L′] are in the range –0.28 to –0.15 V (vs. saturated calomel electrode). Equilibration kinetics of complexation of [RuIII(edta)(H2O)] with L′ have been investigated using stopped-flow methods at 25 °C and pH 5.2. Equilibrium constants determined kinetically are in good agreement with those obtained spectrophotometrically. The reactivity of L′ towards aqua-substitution of [RuIII(edta)(H2O)] complex follows the sequence adenine > adenosine > cytosine > cytidine. On the basis of spectrophotometric, electrochemical and kinetic data and comparing the reactivity of [RuIII(edta)(H2O)] with DNA bases and DNA (calf-thymus) itself it is proposed that the interaction of [RuIII(edta)(H2O)] with DNA takes place through the adenine base unit in a kinetically preferred pathway.

Article information

Article type
Paper

J. Chem. Soc., Dalton Trans., 1995, 2497-2501

Reactivity of [ RuIII(edta)(H2O)] with nucleic bases, nucleosides and DNA (calf-thymus) in aqueous solution (etda = ethylenediamine-N,N,N′,N′-tetraacetate)

D. Chatterjee, H. C. Bajaj and A. Das, J. Chem. Soc., Dalton Trans., 1995, 2497 DOI: 10.1039/DT9950002497

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements