Stability of bis(2,4,6-trichlorophenyl) oxalate in high-performance liquid chromatography for chemiluminescence detection

Abstract

The effects of various factors (organic solvent, type of reservoir, temperature, water content and concentration of hydrogen peroxide) were investigated on the stability of a chemilumigenic reagent, bis(2,4,6-trichlorophenyl) oxalate (TCPO), for high-performance liquid chromatography. In a mixture with hydrogen peroxide, TCPO was most stable when dissolved in acetonitrile and kept in a polyethylene bottle or a high quality glass (borosilicate glass free from metals) flask. The stability of TCPO was also increased by lowering the temperature, the water content and the concentration of hydrogen peroxide in the chemilumigenic reagent solution. Under these conditions, TCPO can be used in a mixed acetonitrile solution with hydrogen peroxide for HPLC-chemiluminescence detection.