Development and optimisation of a high-performance liquid chromatographic assay for tioconazole and its potential impurities. Part II. Selection of detection conditions for potential impurities

Abstract

An optimised high-performance liquid chromatographic separation developed for the assay of tioconazole and its potential impurities has been applied to real-world samples where tioconazole is in excess. As severe peak tailing interferes with the assay of two of the impurity peaks, changes in detection wavelength have been examined as a means to discriminate between this interference. The resulting enhancement of resolution has been exploited in the optimisation of analysis time.