Penicillin biosynthesis: enzymatic synthesis of new cephams
Abstract
Preparations of the enzyme isopenicillin N-synthetase from Cephalosporium acremonium CO 728 convert the modified substrates δ-(L-α-aminoadipoyl)-L-cysteinyl-D-(–)-isoleucine and δ-(L-α-aminoadipoyl)-L-cysteinyl-D-norvaline into cepham-type products, which have been isolated and their structures established.