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Correction: A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation

F. Muttacha, N. Muthmanna, D. Reichertab, L. Anhäusera and A. Rentmeister*ab
aUniversity of Münster, Department of Chemistry, Institute of Biochemistry, Wilhelm-Klemm-Str. 2, 48149 Münster, Germany
bCells-in-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, Germany. E-mail: a.rentmeister@uni-muenster.de

Received 8th November 2017 , Accepted 8th November 2017

First published on 16th November 2017


Correction for ‘A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation’ by F. Muttach et al., Chem. Sci., 2017, DOI: 10.1039/c7sc03631k.


The authors regret that Fig. 4 is incorrect in the original manuscript. In Fig. 4c the chemical structure and mass spectrum of the norbornene-modified adenosine was shown instead of the 2′-deoxyadenosine. The correct figure and caption are displayed below.
image file: c7sc90073b-f4.tif
Fig. 1 Norbornene modification of pBR322 plasmid DNA using the N6-adenine MTase M. TaqI. (A) Scheme for the functionalization of plasmid DNA using norbornene-modified AdoMet analog 1b. (B) Fluorescence labeling of plasmid DNA via norbornene-modification followed by labeling with TAMRA-tetrazine and linearization of the plasmid using BamHI. Bands were resolved on a 1% agarose gel (100 V, 50 min), the gel was stained using ethidium bromide and scanned on a Typhoon FLA9500 laser scanner. (C) Mass spectrometric analysis of N6-norbornene-modified oligonucleotides. A DNA oligonucleotide was subjected to enzymatic norbornene-modification, followed by digestion using nuclease P1 and dephosphorylation using FastAP (ThermoFisher Scientific). Expected mass for C26H31N6O4+ = 491.2401 [M + H]+, found: 491.2400. M: GeneRuler 1 kb DNA ladder (ThermoFisher).

The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.


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