A.
Basso
*a,
C.
Chiorri
b,
F.
Bracco
b,
M. M.
Carnasciali
a,
M.
Alloisio
a and
M.
Grotti
*a
aDepartment of Chemistry and Industrial Chemistry, University of Genova, Italy. E-mail: andrea.basso@unige.it; marco.grotti@unige.it
bDepartment of Educational Sciences, University of Genova, Italy
First published on 23rd February 2018
Improving the interest of high-school students towards chemistry (and science in general) is one of the goals of the Italian Ministry of Education. To this aim, we designed a context-based activity that actively involved students in six different laboratory experiences interconnected by a case study of the murder of Miss Scarlet, from the famous game Clue. Key points of the activity were: the interest aroused by the subject of crime scene investigation; the direct involvement of the students in all stages of the work (from the realization of the experiments to the resolution of the case); the use of a multidisciplinary approach for addressing a complex scientific problem; the work in chemical laboratories with modern instrumentation; the team work and the supervision by young tutors. To verify the hypothesis that such a multidisciplinary activity could foster the interest for the discipline, an evaluation was performed using a self-report questionnaire designed to assess changes in the situational interest raised by the internship. It was found that the activity significantly increased interest and attitude toward chemistry, mainly for students with lower scores in pleasure for the study of chemistry, self-efficacy and self-concept in chemistry.
Improving science education has been high on the political agenda of many European countries since the end of the 1990s. Over the last decade, in particular, a great number of programs and projects have been set up to encourage more students to study science by boosting their interest (Forsthuber et al., 2011). Although it has been recognized that science teaching at primary school has a strong long-term impact, maintaining or recovering high levels of interest is still important at the secondary level, when the likelihood that students will become disengaged with science increases (Dillon and Osborne, 2008).
Many researchers have highlighted that students’ low or declining interest in science is largely due to its presentation as a collection of detached, de-contextualized, and value-free facts that are not connected to their own experiences (Sjøberg, 2002; Osborne et al., 2003; Aikenhead, 2005). Therefore, a potential way of improving student motivation and interest in the subject is to use social and real-life contexts and practical applications as the starting point for the development of scientific ideas (Bennett et al., 2007). This method, referred to as “context-based science teaching” or a “science–technology–society approach”, is considered to increase students’ motivation in scientific studies, and possibly lead to improved scientific achievement (Irwin, 2000; Lubben et al., 2005; Bennett et al., 2007). The effectiveness of a “context-based” learning environment in stimulating pupils’ interest in chemistry has also been reported (Henderleiter and Pringle, 1999; Gutwill-Wise, 2001; Pernaa and Aksela, 2011; O'Dwyer and Childs, 2014).
In Italy, the project “Scientific Degrees Plan”, launched in 2004 and currently ongoing, successfully integrated various private and public institutions, mainly universities, with the aim of promoting science teaching in secondary schools and increasing the number of participants in chemistry, physics, and mathematics degree programs. In this frame, an original “context-based science-teaching” activity has been designed at the Department of Chemistry and Industrial Chemistry of the University of Genova in order to promote the interest of high-school students toward chemistry, by exploiting the great attraction of teenagers for crime scene investigation.
Teaching science through mystery helps students to develop their investigative skills and explore some fascinating hidden science; recently, the initiative “Teaching Enquiry with Mysteries Incorporated” (TEMI) was founded by the European Community with the aim of transforming the way in which Science, Technology, Engineering, and Mathematics (STEM) subjects are taught in classrooms (McOwan et al., 2016). On the other hand, forensic chemistry problems have often been used to stimulate interest in specific chemistry subjects, such as density determination (Saccocio and Carroll, 2006), colorimetric analysis (Ravgiala et al., 2014), thin layer chromatography (Hasan et al., 2008), liquid chromatography (Beussman, 2007), and gas chromatography (Henck and Nally, 2007), or even niche subjects such as Energy-Dispersive X-ray Fluorescence Spectrometry (Palmer, 2011) or Laser Induced Breakdown Spectroscopy (Randall et al., 2013). Laboratory activities associated with these forensic problems, however, were restricted to specific subjects, offering a narrow view of the chemistry world and therefore not being suitable to induce a change in the interest of the students for the discipline. In order to overcome this drawback, instead of specializing in a specific technique, we designed an activity able to include and interconnect all branches of chemistry in a single experience, in order to provide a general overview of what chemistry is and what it can be used for. Moreover, in order to stimulate the interest of the students, we decided to address the following points: (a) the attention aroused by the subject of crime scene investigation and the “playful setting” of the activity; (b) the direct involvement of the student in all phases of the work, from the realization of the experiments to the resolution of the case; (c) the use of a multidisciplinary approach for addressing a complex scientific problem; (d) the opportunity to work in chemical laboratories with modern instrumentation; and (e) the team work and the supervision by young tutors.
After six years of this project, involving about 500 students from various Liguria and southern Piedmont (North Italy) secondary schools, we present here the activity in detail, and its evaluation by a questionnaire.
To find out, twenty-five students per week, divided into teams of five and supervised by young tutors, initially investigated the crime scene, using the techniques of the Scientific Police to detect fingerprints and traces of blood; then they took off several artefacts (pieces of cloth, locks of hair, dust and metal fragments) that were the subject of investigations carried out in the various sections of the department. These investigations allowed students to come into direct contact with different aspects of chemistry and with modern equipment and technologies. Alongside the specific objectives of the various experiences, closely related to the murder case, students had the opportunity to delve into more general topics and see how chemistry is also central in everyday life. The five-day activity ended with a presentation by the students, through which they could describe the applied methodologies and collected data, discuss the deduced information, and substantiate the identification of the culprit.
Forensic analysis consisted of the examination of bloodstains (by a luminol test) and fingerprints (by a ninhydrin test) and involved the students in routine techniques of crime scene investigation. These experiments were useful to critically highlight the difficulties that arise depending on the different materials analyzed and as a result of possible false positives, as well as to discuss more general issues, like the organic reactions characterized by light emission and blood constituents. The experience in the analytical chemistry laboratory introduced the students to the concept of qualitative and quantitative analysis, through the identification of an unknown white powder by classical qualitative tests and flame tests, as well as the quantification of magnesium in the suspects’ hair samples by modern atomic spectrometry. In the industrial chemistry laboratory, qualitative analysis of fabrics discovered on the crime scene was performed. Here, dissolution, distillation and combustion tests and optical microscope observations stimulated the discussion on the comparative properties of natural and synthetic fabrics, structure–property relationships in polymers and plastic recycling. The organic chemistry experience was aimed at identifying the composition of an essential oil, collected from the crime scene and related to the profession of one of the suspects. Steam distillation, liquid–liquid extraction and gas-chromatographic analysis were carried out by students, who could also discuss about separation and purification techniques, natural substances, and small molecule–receptor interactions. Analysis of debris by X-ray diffraction in the physical chemistry laboratory allowed the comparison of an unknown sample with materials used in construction and restoration, stimulating the discussion on crystals and their properties. Finally, elemental and metallographic analysis of metal fragments, besides the identification of the murder weapon, served to discuss about metals and alloys and illustrate the techniques for their characterization. Further details on each experiment are available in Appendix 3.
This final task was an opportunity to highlight their creativity and imagination, as well as the enthusiasm with which they have dealt with the investigation.
Item | Question |
---|---|
1 | I am fascinated by Chemistry |
2 | I chose to take the internship because I’m really interested in the topic |
3 | I am really excited about taking this activity |
4 | I am really looking forward to learning more about Chemistry |
5 | I think the field of Chemistry is an important discipline |
6 | I think that the field of Chemistry will be important for me to know |
7 | I think that the field of Chemistry will be worthwhile for me to know |
8 | When I do Chemistry, I sometimes get totally absorbed |
9 | Because doing Chemistry is fun, I wouldn't want to give it up |
10 | Chemistry is important to me personally |
11 | Most people can learn to be good at Chemistry |
12 | You have to be born with the ability to be good at Chemistry |
13 | I’m confident that I can do an excellent job on my Chemistry tests |
14 | I’m certain I can understand the most difficult material presented in Chemistry texts |
15 | I’m confident I can understand the most complex material presented by my Chemistry teacher |
16 | I’m confident I can do an excellent job on my Chemistry assignments |
17 | I’m certain I can master the skills being taught in my Chemistry class |
18 | How good do you think you are in Chemistry? (not good – very good) |
19 | For me, Chemistry is… (very difficult – very easy) |
20 | Compared to your classmates, how good do you think you are in Chemistry? (the worst – the best) |
Field | Activity | Objectives | Techniques | Discussion issues |
---|---|---|---|---|
Forensic chemistry | Examination of blood stains and fingerprints | Introduce the student in routine analysis carried out on the crime scene | Luminol test; ninhydrin test | Organic reactions characterized by light emission; blood and its constituents |
Analytical chemistry | Qualitative and quantitative analysis of magnesium | Introduce the concepts of qualitative and quantitative analysis | Flame test; colorimetric assays; atomic absorption spectrometry | Sample handling; classic and instrumental analysis; atom structure |
Industrial chemistry | Qualitative analysis of fabrics | Compare the properties of natural and synthetic fabrics | Dissolution, distillation and combustion tests; optical microscopy | Natural and synthetic fabrics; polymers and their properties; plastic recycling |
Inorganic chemistry | Elemental and metallographic analysis of metal fragments | Introduce the notion of metal and alloys and their characterization | X-ray fluorescence; scanning electron microscopy | Analysis of metal surfaces; spectroscopic methods |
Organic chemistry | Determination of the composition of an essential oil | Introduce the use of separation and purification techniques | Steam distillation; liquid–liquid extraction; gas-chromatography | Natural substances; small molecule–receptor interactions |
Physical chemistry | Crystallographic analysis of debris | Introduce the properties of materials used in construction and restoration | X-ray diffraction | Crystals and X-ray diffraction; chemistry for cultural heritage |
Questions 1–7 were aimed to assess the situational interest (Hidi and Renninger, 2006; Linnenbrink-Garcia et al., 2010), considering both feeling-related (1–3) and value-related (4–7) valences (Schiefele, 1991). In order to evaluate possible changes due to the activity, these questions were delivered immediately before and after it. The other questions were administered only before the activity, to assess the pleasure for the study of chemistry (8–10), beliefs about chemistry (11–12), self-efficacy in chemistry (13–17) and self-concept in chemistry (18–20). The items from 8 to 20 were not administered after the experience also because the activity aimed at increasing the situational interest, while the pleasure for the study of chemistry, the beliefs, self-efficacy, and self-concept in chemistry are constructs that were not directly addressed by the internship. Moreover, they are based on items focused on past experiences of study, rather than the current experience at the end of the activity, and whose change is supposed to take place over a long time.
A Wilcoxon test was carried out to test changes in the scores of questions 1–7. After correction for multiple comparisons of the p-value to control for false discovery rate, the test revealed a significant (p < 0.001) change in the scores before and after the experience for questions 1–3. These items are related to the feeling-related situational interest, which refers to the feelings (e.g. enjoyment, involvement) that are associated with the subject, while the value-related interest refers to the attribution of personal significance and importance (Schiefele, 1991). Since there was a substantial correlation among the scores of these three items (mean correlation before the experience: 0.66; mean correlation after the experience: 0.52), the scores of these items were summed to yield a single positive attitude toward the chemistry score (Cronbach's alpha: 0.85 and 0.76, before and after the experience, respectively). These scores allowed us to detect significant changes in each single student using the Reliable Change Index (Jacobson and Truax, 1991), and a new variable (change vs. no change) was created. Twelve (14.2%) students showed a significant increase in scores (and, consequently, in their positive attitude toward chemistry), while no student showed a significant decrease. Then, the association of this new variable with the issues assessed by questions 8–20 was investigated using multi-level linear multiple regression models, which also included as covariates gender, grade, and school type. The details of the statistical analysis results are reported in Appendix 1.
Concerning the pleasure for the study of chemistry, two significant effects were found: the change in the interest (−0.78; p < 0.05) and the school type (+1.07; p < 0.01). The first result means that the students who increased their interest after the activity (hopefully, due to the activity) had lower pleasure for the study of chemistry before the experience than the others, which would indicate a good effectiveness of the proposed activity. In fact, the motivational trigger for this activity was due to its playful and emotionally engaging nature. It is possible that this characteristic had a relevant effect on the students who had a (relatively) poorer motivation for the chemistry and, consequently, a lower pleasure. The activity seemed then able to move them from a poor and extrinsic motivation (“I study it because I have to”) to a motivation based on incentives (“I study it because I am having fun”). On the other hand, the students that already had an intrinsic motivation towards chemistry (“I study it because I like it”) were less prone to show potential positive effects due to emotions triggered by the playful context. The incentive based on positive emotions is particularly effective in those who have a poor motivation, while its effects are mild or even absent in students who already have an intrinsic motivation, who find the pleasure in the subject itself.
The second result indicates a greater pleasure for the study of chemistry for students from technical institutes compared to high-school students, but the implications of this result are beyond the purpose of this work.
Concerning the beliefs about chemistry, no significant effect was found, while both self-efficacy and self-concept in chemistry were significantly related to the change in the interest (−0.62 and −0.51; p = 0.055 and p = 0.030 respectively). Again, these results suggest that the improvement in the interest occurred in students with lower self-efficacy and self-concept in chemistry than the others.
In this context, the proposed activity showed a possible solution to successfully raise the interest of high-school students for chemical science in its various aspects, comprising analytical, inorganic, physical, organic, and industrial chemistry. It emphasized multidisciplinary aspects and focused on the interest aroused by the subject of scientific investigation of the crime scene and on the direct involvement of the student in all phases of the work, i.e. the realization of the experiments, the discussion of the results, possible explanations and implications, the presentation of the conclusions and the resolution of the case. Moreover, it was made apparent to them that a given scientific problem needs to be faced from different points of view and with high critical thinking, in order to come to a sound conclusion.
Specifically, data from a self-report questionnaire assessing interest and attitude toward chemistry, pleasure for the study of chemistry, beliefs about chemistry, self-efficacy in chemistry, and self-concept in chemistry showed that after the activity there was a general significant increase in interest and attitude. We also found that students who showed a significant change (increase) in this variable were those who had lower scores in pleasure for the study of chemistry and self-efficacy and self-concept in chemistry before the activity. Overall, the results of this study suggest that the activity can be especially effective on those students that show relatively lower levels of positive attitude and self-efficacy in chemistry. Future studies could investigate whether these students differ from their peers in other variables relevant to school achievement, such as motivation and/or learning styles. The playful and emotionally engaging context could have changed the students’ motivation from poor and extrinsic to one based on incentives, the positive emotion being the reward for studying chemistry. A further step, after this activity, would be directed toward the development of an intrinsic motivation, where the pleasure for the study of chemistry is no longer dependent on the playful context, but is found in the subject itself.
The experiences have always been challenging and exciting, as demonstrated by the answers to the questionnaire filled in by the students at the end of the internship. A limitation of this result could be the use of self-report measures, which could have introduced some well-known potential biases of questionnaire measures, such as socially desirable and acquiescent responding. The anonymous completion of questionnaires should have controlled these biases, but a more conclusive test of the efficacy of the activity described here could be obtained by comparing objective chemistry achievement before and after the activity, as it would also shed light on its long-term effect.
Estimate | Std error | df | t value | Pr(>|t|) | |
---|---|---|---|---|---|
Note: significant effects are indicated in bold. | |||||
Intercept | 4.122 | 1.300 | 75.00 | 3.171 | 0.0022 |
Interest | −0.782 | 0.392 | 75.00 | −1.994 | 0.0498 |
School type | 1.073 | 0.396 | 75.00 | 2.709 | 0.0084 |
Gender | 0.438 | 0.281 | 75.00 | 1.555 | 0.1242 |
Grade | −0.004 | 0.316 | 75.00 | −0.013 | 0.9899 |
Estimate | Std error | df | t value | Pr(>|t|) | |
---|---|---|---|---|---|
Intercept | 2.933 | 0.979 | 76.00 | 2.995 | 0.0037 |
Interest | 0.149 | 0.286 | 76.00 | 0.521 | 0.6042 |
School type | 0.038 | 0.298 | 76.00 | 0.128 | 0.8985 |
Gender | 0.093 | 0.212 | 76.00 | 0.441 | 0.6606 |
Grade | 0.207 | 0.238 | 76.00 | 0.867 | 0.3889 |
Estimate | Std error | df | t value | Pr(>|t|) | |
---|---|---|---|---|---|
Note: significant effects are indicated in bold. | |||||
Intercept | 4.913 | 1.124 | 75.92 | 4.372 | 3.85 × 10−5 |
Interest | −0.620 | 0.318 | 70.81 | −1.949 | 0.0553 |
School type | 0.464 | 0.329 | 68.04 | 1.412 | 0.1625 |
Gender | 0.359 | 0.235 | 70.14 | 1.527 | 0.1313 |
Grade | −0.235 | 0.274 | 75.92 | −0.858 | 0.3934 |
Estimate | Std error | df | t value | Pr(>|t|) | |
---|---|---|---|---|---|
Note: significant effects are indicated in bold. | |||||
Intercept | 5.047 | 0.803 | 75.96 | 6.284 | 1.9 × 10−8 |
Interest | −0.505 | 0.228 | 70.52 | −2.217 | 0.0298 |
School type | −0.012 | 0.236 | 67.40 | −0.051 | 0.9593 |
Gender | 0.056 | 0.168 | 69.72 | 0.334 | 0.7392 |
Grade | −0.075 | 0.196 | 75.97 | −0.385 | 0.7015 |
Question 1: how can you determine the date of death of a corpse found after several days?
(a) from the body temperature; (b) by entomological analysis; (c) from the rigidity of the body (rigor mortis).
Question 2: which chemical elements are searched for in the analysis of residues of gun powder?
(a) Sb–Pb–Ba; (b) Pb–Cu–Zn; (c) Pb–C–Ti.
Question 3: when can you say that two fingerprints match?
(a) when they are perfectly superposable; (b) when they display a certain number of coincident “unique regions”; (c) when they have the same number of “ridges” and “grooves”.
Question 4: on what kind of reaction are explosives generally based?
(a) combustion; (b) hydrolysis; (c) self redox.
Question 5: what pathological criterion indicates a strychnine poisoning?
(a) early stiffness; (b) carmine red hypostasis; (c) early putrefaction.
Question 6: what is the active ingredient of Ecstasy?
(a) lysergic acid diethylamide; (b) 3,4-methylenedioxymethamphetamine; (c) delta-9-tetrahydrocannabinol.
Question 7: which metallic element is present in the heme group of hemoglobin?
(a) Ca; (b) Fe; (c) Em.
Question 8: what is the difference between deflagration and detonation?
(a) in the detonation expansion is supersonic; (b) the explosion causes more extensive damage; (c) the detonation requires a trigger device.
Question 9: how samples are collected in the analysis of residues of gun powder?
(a) by means of adhesive pads; (b) on a photographic plate; (c) with micro-tweezers.
Question 10: bleach (sodium hypochlorite) is:
(a) an acid; (b) an oxidant; (c) a buffer.
Question 11: A girl drinks caustic soda, erroneously dissolved in mineral water. The acid caused her severe burns. Where is the mistake?
(a) caustic soda is not an acid; (b) caustic soda does not cause burns; (c) caustic soda cannot be dissolved in mineral water.
Question 12: which wine sophistication causes its high toxicity?
(a) addition of ethyl alcohol; (b) addition of methanol; (c) addition of sucrose.
– Monday morning: presentation of the case study, assignment of participants to groups 1–5 and recruitment test.
– Monday afternoon: analysis of evidence with luminol and ninhydrin.
– Tuesday morning and afternoon, Wednesday morning: three distinct laboratory experiences.
– Wednesday afternoon: preliminary elaboration of data.
– Thursday morning and afternoon: two distinct laboratory experiences.
– Friday morning: final elaboration of data and preparation of the presentation.
– Friday afternoon: presentation and conclusion.
The victim is Kassandra Scarlet, 25, an office secretary and artistic restoration aficionado. On the evening of the murder she left work in a hurry because of a severe toothache.
The suspects are:
(1) Jacob Green, 30, brother of the victim, a butcher, the sole heir of the family assets in the event of the death of his sister.
(2) Victor Plum, 28, partner of the victim, a mason, a weightlifting enthusiast, had a violent quarrel with the girl the night before the murder for reasons of jealousy.
(3) Diane White, 27, a friend of the victim, employed in a grocery store, ex-girlfriend of Victor Plum and envious of her beauty.
(4) Jack Mustard, 52, a neighbor, a worker in a steel industry, with a criminal record of stalking of the victim.
(5) Eleanor Peacock, 35, a colleague of the victim, had a heated argument a few days before the murder.
Activity | Evidence | Suspected | Exonerated |
---|---|---|---|
Luminol test | A; F1–F10 | — | — |
Ninhydrin test | M | 1-2-3 | — |
Analytical chemistry | B; H1–H5 | 2-3-4 | 1-5 |
Organic chemistry | E | 3 | — |
Inorganic-metallurgical chemistry | A, G, N | 4 | 1 |
Physical chemistry | D, L | — | 2 |
Industrial chemistry | C; I1–I5 | 3-5 | 1-2-4 |
Finally, several procedures made use of high-level instruments, which are not usually available in high schools. However, this should not be seen as a weakness but rather as a strong point of the proposed activity, which was designed to be carried out inside the University, in order to create a connection between educational institutions and allow students to get in contact with modern techniques and advanced methodological approaches.
The test solution is prepared as follows: in a 100 mL beaker weigh 1.5 g of KOH and 0.2 g of luminol, dissolve in 25 mL of deionized water, and then add 10 mL of hydrogen peroxide 3% solution. The test solution is used to analyze exhibits A and F1–F10. Exhibits A and F2 and F3 are preliminarily treated with bleach.
The ninhydrin solution is prepared as follows: in a 100 mL volumetric flask weigh 0.4 g of ninhydrin and dissolve it in 2 mL of methanol, then add 7 mL of ethyl acetate and 1 mL of acetic acid and make up to the volume with petroleum ether.
The semi-burnt envelope (preliminarily impressed with various fingerprints) is cut into pieces and dipped into the ninhydrin solution; then they are transferred into a stove at 90 °C for 10 minutes. The students select then the fragments containing fingerprints.
The second part of this activity is focused on the quantitative analysis of exhibits H1–H5. Weigh precisely 20–50 mg of each sample and put them into 5 flasks. Under a fume hood, add 20 mL of HNO3 1:1 (v/v) and heat it to the boiling point on a hot plate. Transfer the solutions into 50 mL volumetric flasks and make up to the volume with deionized water. Prepare a series of standard magnesium solutions by diluting a stock Mg solution 1000 ppm. Analyze the standards and samples using atomic absorption spectrometry (samples 2-3-4 have to be furtively spiked with a magnesium standard solution to obtain a high concentration).
Steam distillation: place the cotton ball in a 100 mL round bottom flask, add 60 mL of distilled water and build the distillation apparatus. Heat it until boiling and distill it into test tubes until the distillate is clear, indicating that there is no essential oil left on the cotton ball.
Liquid–liquid extraction: transfer the test tubes containing the essential oil (opalescent solution) into a separatory funnel, add ethyl acetate (20 mL) and separate the phases. Collect the organic phase and re-extract the water phase with ethyl acetate (20 mL). The combined organics are anhydrified and concentrated under reduced pressure. The resulting oil is used for qualitative analysis.
GC-MS analysis: weigh 1 mg of the resulting oil in a vial and dissolve it in 1 mL of diethyl ether. Perform gas chromatographic analysis with an instrument coupled with a mass spectrometer. Observe the peaks on the chromatogram and identify the corresponding substances through their fragmentation pattern, with the aid of a database.
Metallographic samples of exhibits A and G are prepared by incorporation into resin, then polished and observed under an optical microscope. Subsequent metallographic attack of the surface put in evidence the crystal structure. Thanks to scanning electron microscopy equipped with an elemental X-ray micro-analyzer, it is possible to observe the true nature of the two exhibits, and recognize the fragment found in the back of the victim as a chrome-plated steel and not a stainless steel.
Exhibits D and L are then analyzed by X-ray diffraction of powders. By comparison between the spectra obtained from the two samples, the different compositions and crystal structures are highlighted.
Microscopic analysis: separation of warp and weft fibers is followed by their deposition on a microscopy glass slide, by dipping them in one drop of paraffin. Observation and image recording are performed.
Proof of combustion: bring the fibers (warp and weft separately) to the open flame and observe how the combustion proceeds, the aspect of the unburned residue and the smell released during combustion.
Dry distillation: insert the fibers (warp and weft separately) in test tubes and place a piece of moistened litmus paper on them, place the tubes on the open flame until the fibers start to melt and release vapors. Observe the color variation of the paper and determine the pH of the vapors.
Treatment with solvents: introduce a predefined amount (about 30 mg) of fibers (warp and weft separately) in a test tube and perform the following operations in succession:
(1) add 3 mL of acetone, heat to boiling for 5 min and note the results;
(2) if the fabric does not dissolve, remove the acetone with a pipette, add 3 mL of glacial acetic acid, heat to boiling for 2–3 minutes, and note the results;
(3) if the fabric does not dissolve, remove the acetic acid using a pipette, add 3 mL of nitrobenzene and note the results;
(4) if the fabric does not dissolve, remove the nitrobenzene with a pipette, add 3 mL of 10% KOH and note the results;
(5) if the tissue dissolves, add HCl at room temperature and note the results;
(6) if the fabric does not solubilize, take note of the result.
Further experimental details are available on request by the authors.
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