Salma
Mumtaz
ab,
Israel
Cano
*b,
Nargis
Mumtaz
a,
Ahmed
Abbas
c,
Jairton
Dupont
*bd and
Humaira Yasmeen
Gondal
*a
aDepartment of Chemistry, University of Sargodha, Sargodha, 40100, Pakistan. E-mail: hygondal@yahoo.com
bGSK Carbon Neutral Laboratory for Sustainable Chemistry, University of Nottingham, NG7 2GA, Nottingham, UK. E-mail: israel.canorico@nottingham.ac.uk
cH.E.J. Research Institute of Chemistry, ICCBS, University of Karachi, Karachi, 75270, Pakistan
dLaboratory of Molecular Catalysis, Institute of Chemistry, UFRGS, Av. Bento Gonçalves, 9500, Porto Alegre 91501-970, RS, Brazil. E-mail: jairton.dupont@ufrgs.br
First published on 23rd July 2018
A series of novel benzimidazolium-based non-racemic ionic liquids (ILs) was synthesized from low-cost chiral terpenoid alcohols and fully characterized by the use of a wide variety of techniques, such as DSC, ESI-MS, ATR FT-IR, polarimetry as well as 1H and 13C NMR spectroscopy. The ILs were investigated as chiral shift agents for the chiral recognition of racemic mixtures of Mosher's acid potassium salt by 19F NMR spectroscopy, leading to high splitting values of the CF3 signal. Supramolecular interactions between salt and H–C2 of chiral benzimidazolium cation are responsible for the chiral recognition, as was demonstrated by experimental evidences. Indeed, the enantiomeric excess value of enantioenriched substrates depends mainly on the strength of the contact ion pairs.
Therefore, in order to contribute towards the understanding upon the supramolecular interactions that can be established between racemic substrates and non-racemic ILs, we have designed and prepared a new family of chiral ILs and explored their potential use as chiral recognition agents. Herein we report the synthesis of a new family of benzimidazolium-based chiral ILs prepared from commonly available and inexpensive chiral terpene alcohols such as (+)-menthol, (+)-fenchyl alcohol and (+)-isopinocampheol (Fig. 1). The obtained enantiomerically pure ILs were characterized by DSC, ATR FT-IR, ESI-MS, polarimetry along with 1H and 13C NMR spectroscopy. In addition, the new ILs were studied as chiral shift agents for the chiral recognition of racemic mixtures of Mosher's acid potassium salt. Experimental studies enabled us to elucidate the nature of the interaction between the non-racemic ILs and the racemic Mosher's acid potassium salt. Finally, the ILs demonstrated their ability for the determination of the enantiomeric excess of enantioenriched samples.
Fig. 1 Natural secondary alcohols 1a: (+)-menthol; 1b: (+)-fenchyl alcohol; 1c: (+)-isopinocampheol. |
The obtained non-racemic ILs 4–7 were completely characterized by Fourier-Transform Infrared (ATR FT-IR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and 1H and 13C NMR spectroscopy (for further details see ESI†), whereas their melting point was studied by differential scanning calorimetry (DSC). Table 1 displays the most important physical properties of 4–7.
Entry | Non-racemic IL | Physical stateb | Yield (%) | Mpc (°C) | [α]20Dd (deg mL g−1 dm−1) |
---|---|---|---|---|---|
a Uncertainties of measured values: yield, ±1%; mp, ±0.5 °C; [α]20D, ±2%. b Physical state at 20 °C. c Mp: melting point determined by DSC. d [α]20D: c = 4 mg mL−1, MeOH, 20 °C. | |||||
1 | 4a | White solid | 97 | 127 | +136.5 |
2 | 5a | White solid | 99 | 132 | +147.5 |
3 | 6a | White solid | 99 | 123 | +94.5 |
4 | 7a | Light brown oil | 99 | — | +96.5 |
5 | 4b | White solid | 95 | 124 | +39.8 |
6 | 5b | White solid | 98 | 122 | +38.2 |
7 | 6b | White solid | 99 | 147 | +45.0 |
8 | 7b | Light brown oil | 99 | — | +22.5 |
9 | 4c | White solid | 93 | 122 | +37.8 |
10 | 5c | White solid | 98 | 124 | +41.0 |
11 | 6c | White solid | 99 | 124 | +55.0 |
12 | 7c | Light brown oil | 98 | — | +30.0 |
All synthesized ILs are non-racemic, as it is confirmed by their specific rotation ([α]20D). We observed a trend in the [α]20D values of ILs depending on the chiral building block ((+)-menthol, 1a; (+)-fenchyl alcohol, 1b; (+)-isopinocampheol, 1c) employed for their synthesis. In the case of (+)-menthol ILs 4a–7a, there is a large difference between the specific rotation of Cl− and BF4− based salts and those of PF6− and NTf2− ILs (Fig. 2). However, for 4b–7b and 4c–7c the [α]20D values of Cl− based salts were found to be similar to those of BF4− and lower than PF6− based non-racemic ILs, whereas NTf2− compounds showed the lowest values of [α]20D (Fig. 2). On the other hand, DSC revealed a melting point (mp) in the range of 122–147 °C for 4–6 (Table 1), while all NTf2 ILs are liquid at RT.
Different concentrations of 6b regarding the racemic salt were tested in order to find the optimal amount of chiral IL required for maximum splitting of CF3 signal of racemic Mosher's acid potassium salt. The influence of 6b concentration on the degree of CF3 signal splitting is represented graphically in Fig. 3. Two equiv. of 6b was the best ratio to obtain the maximum splitting (23.7 Hz, Fig. 3), whereas the extent of the splitting decreased for higher concentrations of 6b. The influence of IL concentration on the magnitude of chemical shift difference is well known and has been previously described by several authors.5d,15,17
Fig. 3 19F NMR chemical shift (recorded in toluene-d8) of racemic Mosher's acid potassium salt in the presence of different amounts of 6b. |
The nature of the interaction between the chiral ILs and the racemic Mosher's acid potassium salt was investigated by experimental studies. The racemic salt could interact with the IL through hydrogen bonding with the H–C2 of the enantiopure imidazolium cation.18 However, an interaction by π–π stacking between the phenyl groups of both compounds is also likely.4b Therefore, we prepared the chiral ILs 8 and 9 in order to investigate the type of interaction between the different species in solution (Fig. 4).
Benzimidazolium-based chiral IL 4a led to a splitting of 19.6 Hz in toluene (Fig. 5), whereas the use of 8 resulted in a similar discrimination (Fig. 5, 19.9 Hz). Consequently, the formation of the diastereomeric adduct is not attributable to the π–π stacking interactions between the phenyl groups of racemic substrate and chiral IL. Interestingly, no splitting was observed with 9 (Fig. 5), for which the hydrogen H–C2 was replaced by a methyl group. This proves that the chiral IL interact with the Mosher's acid potassium salt through hydrogen bonding with the hydrogen atom located in C2 position of the imidazolium ring. This is one of the rare cases in which clear experimental evidences of the supramolecular interactions responsible for chiral recognition are observed.
Fig. 5 19F NMR investigation of the interaction between the non-racemic ILs and the racemic Mosher's acid potassium salt by the use of different imidazolium-based ILs. |
Next, we studied the role of solvents and nature of chiral ILs on the extent of splitting of CF3 signal, since the strength of ion pairs are dependent on the polarity of the solvent employed.19 The experiments were conducted using different deuterated solvents but splitting was only observed in CDCl3 and toluene-d8 (Table 2), while no splitting was detected when more polar solvents such as D2O, MeOD and CD3CN were used. Consequently, it was realized that the solvent strongly affects the formation of the diastereomeric adduct and a reduction in its polarity probably increase the strength of the hydrogen bonding (and contact ion pair)20 and thus the interaction between the molecules of IL and Mosher's acid derivative.18 In most cases, changing the solvent to toluene leads to an increase in splitting of the 19F NMR signal of the racemic Mosher's acid potassium salt in comparison with CDCl3 (Table 2). For instance, chiral ILs 4–5a and 5–6b produced a splitting >8 units higher in toluene than those obtained in CDCl3 (entries 1 and 2). Similarly, the use of chiral ILs 5c and 6c resulted in a splitting of 15.3 and 8.6 Hz in toluene, respectively, whereas no splitting was observed when CDCl3 was utilized as solvent (entries 10 and 11).
Entry | Non-racemic IL | Precursor alcohol | X | Δδb (Hz) | |
---|---|---|---|---|---|
CDCl3 | Toluene | ||||
a Reagents and conditions: non-racemic IL (2 equiv.), racemic Mosher's acid potassium salt (1 equiv.), deuterated solvent (0.6 mL). NS: no splitting. b Uncertainty for Δδ: ±2%. | |||||
1 | 4a | (+)-Menthol | Cl | 9.0 | 19.6 |
2 | 5a | (+)-Menthol | BF4 | 10.6 | 19.0 |
3 | 6a | (+)-Menthol | PF6 | 11.6 | 11.3 |
4 | 7a | (+)-Menthol | NTf2 | NS | NS |
5 | 4b | (+)-Fenchol | Cl | 7.2 | 15.0 |
6 | 5b | (+)-Fenchol | BF4 | 9.7 | 19.0 |
7 | 6b | (+)-Fenchol | PF6 | 12.3 | 23.7 |
8 | 7b | (+)-Fenchol | NTf2 | NS | NS |
9 | 4c | (+)-Isopinocampheol | Cl | 11.3 | 11.5 |
10 | 5c | (+)-Isopinocampheol | BF4 | NS | 15.3 |
11 | 6c | (+)-Isopinocampheol | PF6 | NS | 8.6 |
12 | 7c | (+)-Isopinocampheol | NTf2 | NS | NS |
The magnitude of the splitting is also affected by the type of anion forming the chiral IL used, and it appears to be related with the strength of the contact ion pair. In general, Cl−, BF4− and PF6− based salts form relatively strong ion pairs21 and provided a good discrimination, whereas no splitting was observed for hydrophobic ILs prepared with NTf2− as counteranion, which possess a weaker cation–anion interaction (entries 4, 8 and 12). Regarding the cation, chiral ILs derived from (+)-menthol and (+)-fenchol led to a similar splitting of the 19F NMR signal (entries 1–8), while the values observed for ILs prepared from (+)-isopinocampheol were lower (entries 9–12). In this context, the maximum splitting of CF3 signal was obtained for 6b (derived from (+)-fenchol), with Δδ = 23.7 Hz (entry 7). This could be due to the higher steric impediment produced by the proximity of methyl groups of fenchol to the benzimidazolium ring in comparison with menthol and isopinocampheol.
The high chiral discrimination induced by ILs 4–7 encouraged us to evaluate their use for the determination of the enantiomeric excess of enantioenriched samples of Mosher's acid salt. Two samples with approximately R/S = 2:1 and R/S = 4:1 were prepared through the addition of (R)-Mosher's acid salt to the corresponding racemic salt. The integration of CF3 signals observed in the 19F NMR spectra provided the almost exact ratio of each enantiomer in the samples (Fig. 6). Consequently, the ILs described herein can be successfully employed to determine the ee value of chiral samples.
Fig. 6 19F NMR spectra and determination of % ee values of enantioenriched samples of Mosher's acid potassium salt. (a) R/S = 2:1, (b) R/S = 4:1. |
Footnote |
† Electronic supplementary information (ESI) available: General experimental procedures, synthetic procedures, characterization of new compounds, and 1H NMR and 13C NMR spectra. See DOI: 10.1039/c8cp03881c |
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