Issue 18, 2018

One-step detection of hexokinase activity using a personal glucose meter

Abstract

Hexokinase (HK) is the first key enzyme in metabolic and glycolytic pathways. Aberrations in HK activity may result in various human diseases. In this study, we present a novel, one-step, simple, and rapid method for monitoring HK activity using a commercial personal glucose meter (PGM). In this design, HK catalyzes glucose phosphorylation, forming phosphorylated glucose, which is not detected by a PGM. Accordingly, the amount of unphosphorylated glucose remaining in solution following the HK-catalyzed reaction, which is inversely related to HK activity, can be determined using a PGM. Owing to the favorable features of the PGM, such as its simple operation, small size, low cost, wide accessibility, and portability, this proposed assay has great potential for the point-of-care testing of HK activity.

Graphical abstract: One-step detection of hexokinase activity using a personal glucose meter

Article information

Article type
Communication
Submitted
08 Mar 2018
Accepted
09 Apr 2018
First published
10 Apr 2018

Anal. Methods, 2018,10, 2075-2080

One-step detection of hexokinase activity using a personal glucose meter

J. Bai, L. Liu, Y. Han, C. Jia and C. Liang, Anal. Methods, 2018, 10, 2075 DOI: 10.1039/C8AY00498F

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