Amide rotation trajectories probed by symmetry

Vladimir Kubyshkin * and Nediljko Budisa *
Biocatalysis Group, Institute of Chemistry, Technical University of Berlin, Müller-Breslau-Str. 10, Berlin 10623, Germany. E-mail: kubyshkin@win.tu-berlin.de; nediljko.budisa@tu-berlin.de

Received 12th June 2017 , Accepted 22nd July 2017

First published on 24th July 2017


Amide rotation of peptidyl–prolyl fragments is an important factor in backbone structure organization of proteins. Computational studies have indicated that this rotation preferentially proceeds through a defined transition-state structure (syn/exo). Here, we complement the computational findings by determining the amide bond rotation barriers for derivatives of the two symmetric proline analogues, meso and racemic pyrrolidine-2,5-dicarboxylic acids. The rotations around these residues represent syn/exosyn/exo and anti/endosyn/exo hybrid transition states for the meso and racemic diastereomer, respectively. The rotation barriers are lower for the former rotation by about 9 kJ mol−1 (aqueous medium), suggesting a strong preference for the syn/exo (clockwise) rotation over the anti/endo (anticlockwise) rotation. The results show that both hybrid rotation processes are enthalpically driven but respond differently to solvent polarity changes due to the different transition state dipole–dipole interactions.


Introduction

Proline (1, Fig. 1A) plays an important role in various areas of organic and biological chemistry. These include cellular uptake,1 metabolism,2 translation,3 protein folding and engineering,4 organocatalysis,5 pre-biotic chemistry,6 foldamer research,7 drug design,8 and more. A particularly interesting focus is the tertiary amide bond formed by peptidyl–prolyl fragments in peptide and protein structures.9,10 The isomerization of this backbone structure can often become the rate-limiting step in protein folding.11 This process is frequently facilitated by peptidyl–prolyl cistrans isomerases in natural systems12 alternatively by substitution of proline with its analogues in laboratory protein expression experiments.13 Notably, peptidyl–prolyl isomerization is not necessarily slow compared to other amide bond rotations; rather, it may become rate limiting due to the relative thermodynamic stability of the non-native versus the native state.14
image file: c7ob01421j-f1.tif
Fig. 1 (A) The structure of proline (1), (B) two N-acyl-prolyl ground states, s-trans and s-cis, (C) four possible transition states for the amide rotation, the syn/exo transition state is most favored. The nomenclature is from ref. 21.

The two ground states of the N-acyl prolyl amide bond are s-trans and s-cis (Fig. 1B), with s-trans conformation forming preferentially. The preference is about 3[thin space (1/6-em)]:[thin space (1/6-em)]1 in Ac-Pro-NHMe,15 4[thin space (1/6-em)]:[thin space (1/6-em)]1 in Ac-Pro-OH,16 5[thin space (1/6-em)]:[thin space (1/6-em)]1 in Ac-Pro-OMe,17 and 7[thin space (1/6-em)]:[thin space (1/6-em)]1 in GlyProGly-NH218 and AcGlyGlyProGlyGlyNH2 peptides (water, 298 K),19 which translates to approximately 4–5 kJ mol−1 in energetic preference. At the same time, the amide bond rotation should proceed through one of the four theoretically possible transition states, in which the amide conjugation should be lost (Fig. 1C). Several structures have successfully depicted the non-conjugated state trapped by a rigid molecular scaffold (so-called twisted amides).20 However, these elegant structures do not definitively model the amide transition state due to the following reason. Already in the mid-1990s the results from a theoretical study showed that the amide rotation preferably proceeds via the syn/exo transition states with the antiparallel orientation of the N → lone pair and carbonyl C → O dipoles,21 whereas in the covalent twisted amides this orientation rather resembles a parallel one. Only very recently metal coordination based twisted amides have been reported, and these demonstrated the antiparallel twist.22

Since the seminal theoretical reports, numerous computational studies have demonstrated that the amide rotation around proline has a preference for the syn/exo rotation scenario.23 These findings are further supported by recent theoretical24 and experimental25 studies of the catalytic mechanism of Pin1 peptidyl–prolyl cistrans isomerase, and experimental demonstration of the self-catalytic mechanism of the amide rotation in nonpolar media.26

In the syn/exo transition state, the nitrogen lone pair is oriented on the same side and the carbonyl oxygen rotates into the opposite side with respect to the proline carbonyl group, whereas the upstream alkyl moiety is oriented outward. Curiously, despite the existing consensus among theoretical studies, a proper experimental model that addresses the energetic differences in the relevant rotation modes is lacking. While there is little doubt that the syn/exo transition state is the most relevant, it is not clear whether the anti/endo transition state can contribute to the rotation or whether the properties of the syn/exo conformation can be extrapolated to other transition scenarios. Therefore we designed an experimental model based on derivatives of pyrrolidine-2,5-dicarboxylic acid (Pdc). Pdc has two diastereomeric forms, meso (mPdc, with a symmetry plane, σ) and racemic (rPdc, with a symmetry axis, C2). We assumed that the amide ground states are equivalent in the N-acyl Pdc derivatives when the acyl-moiety is not chiral. At the same time, we expected the amide rotation to proceed via the syn/exosyn/exo hybrid state for mPdc and the anti/endosyn/exo hybrid state for rPdc derivatives (Fig. 2) (using the proline transition state nomenclature, Fig. 1C). We therefore synthesized a series of amides with non-chiral N-terminal acyl groups (Fig. 3). Here, we report the experimental amide bond rotation barriers for these two model situations. The results obtained for proline and pyrrolidine derivatives will also be shown for reference but will not be discussed in the context of this paper.


image file: c7ob01421j-f2.tif
Fig. 2 Design of the study. Two diastereomeric forms of pyrrolidine-2,5-dicarboxylic acid yield different rotation modes around the N-acyl derivatives.

image file: c7ob01421j-f3.tif
Fig. 3 Examined compounds.

Results and discussion

The ground state

We designed our study with the assumption that the amide ground state formed by the Pdc residue will experience similar chemical environments in both diastereomeric situations. The similarity in the through-bond electronic environment of the N-terminal fragment can be assumed from the acidity of the amino acid ammonium salts, which was nearly identical for both Pdc derivatives, as shown in Fig. 4.
image file: c7ob01421j-f4.tif
Fig. 4 The pKa of the ammonium group in the pyrrolidine-2,5-dicarboxylic acid derivatives. The data on pyrrolidine and proline methyl esters are included for comparison. Standard error: ±0.10.

Next, the X-ray crystal structures for both N-acetyl derivatives 2a and 3a demonstrate fully planar amide bonds (Fig. 5A).27 In the mPdc derivative 2a, the amide conformation breaks the symmetry of the structure and creates two enantiomers, which were present in a ratio of 4[thin space (1/6-em)]:[thin space (1/6-em)]2 in the cell unit. In the rPdc derivative 3a, the amide does not break the existing C2 symmetry of the structure, and the cell unit contains a racemate with a 2[thin space (1/6-em)]:[thin space (1/6-em)]2 ratio of the (R,R) and (S,S) enantiomers. The envelope conformation of the pyrrolidine ring in proline is usually described in terms of exo-/endo-pucker nomenclature (alternatively called up-/down-) depending on the direction of the C4-atom displacement from the plane of the ring relative to the carboxyl group orientation. Following this nomenclature, the mPdc derivative 2a adopts a hybrid of exo- and endo-pucker, whereas the rPdc derivative 3a is a hybrid of two endo-puckers. The crystal structure is consistent with the solution 1H NMR spectra, in which the two CH(CO2Me) resonances appear as a triplet and doublet of doublets in 2a and as two doublets in 3a (Fig. 5B).28 Importantly, these NMR spectral signatures persisted in all examined Pdc derivatives and in all employed NMR solvents (see ‘Polar effects’).


image file: c7ob01421j-f5.tif
Fig. 5 The X-ray crystal structures (A) and solution 1H NMR (in D2O) data (B) for the N-acetyl compounds 2a and 3a. The ellipsoids represent 30% probability displacement, carbon – black, oxygen – red, and nitrogen – blue.

Based on this spatial organization argument we expect the steric requirements to be identical around the methyl group of the acetyl moiety, whereas the environment of the carbonyl group is different. These differences are very well described in the proline literature in terms of the pre-organization of the n → π* interaction between the carbonyl groups.29 As a result, some additional stability of about 1–3 kJ mol−1 can be expected for the mPdc derivatives relative to the rPdc-derived compounds irrespective from the polarity of the medium.17

Another aspect is the ψ-torsion due to the rotation of the carboxyl-group. For the proline derivative this rotation yields two states ψ1 ∼170° and ψ2 ∼−20° with different polarities. In 5a the first state is preferred by about 1.3–2.5 kJ mol−1.30 We modelled the carboxyl-group rotation in 2a–3a, and found that this preference remains nearly identical for the rPdc derivative 3a (ΔΔGψ1/ψ2∼ 1.6–2.7 kJ mol−1), whereas in 2a the two carboxymethyl groups are more proximal, and the rotation state difference becomes more prominent (ΔΔGψ1/ψ2∼ 3.7–4.7 kJ mol−1). This, however, produces only a little energy difference between the two ground states, and the resulting polarity differences are expected to become influential only in nonpolar media.30

Hereafter, we will not correct for these energies and will consider both ground states equivalent for simplicity of presentation.

Energetic terms of the amide rotation

We then analyzed the amide rotation barriers in the N-acetyl derivatives 2a, 3a and 4a in aqueous medium by 1H EXSY NMR. The results are shown in Fig. 6, where the activation energy values E are presented as a function of temperature (also see Table S1). As expected, the rotation barrier for the mPdc derivative 2a was remarkably lower compared to the rPdc derivative 3a (by 9.3–9.5 kJ mol−1; equivalent to a factor of 43–46 at 298 K).
image file: c7ob01421j-f6.tif
Fig. 6 Amide rotation barriers for the N-acetyl derivatives 2a, 3a and 4a as a function of temperature, measured by 1H EXSY NMR in deuterium oxide. For details, see Tables S1 and S3.

For all three examined structures, the amide rotation is an enthalpically defined process with a small negative slope, −TΔSS > 0). The smallest entropic contribution was found for pyrrolidine derivative 4aS = +3.2 ± 1.8 J mol−1 K−1), whereas for the mPdc derivative 2a, this value was somehow larger (ΔS = +11.9 ± 0.9 J mol−1 K−1). We were not able to determine the entropic value for the rPdc derivative 3a with the same level of accuracy (ΔS = +7.6 ± 9.0 J mol−1 K−1) due to the high relative experimental error for the very slow rates under the lower temperature conditions. Nonetheless, this lack of accuracy does not affect the overall conclusion, as the same tendencies were found for the N-acetylglycyl derivatives 2c–4c. In the latter case, the activation enthalpy was reduced by 5–7 kJ mol−1 and the entropy was reduced by 6–9 J mol−1 K−1 relative to the N-acetyl derivatives (see Tables S2 and S3).

A small positive activation entropy is also known for the amide rotation in the proline derivative 5a, where ΔS has been reported to be +9.0 for cistrans and +12.8 J mol−1 K−1 for transcis rotations, respectively.31,32 The positive entropy of the amide rotation process may originate from the higher molecular rigidity of the ground state (featured by the n → π* interactions, for instance),17,29 as well as the stronger polar interactions of the ground state with water (organization of the solvent). For example, negative activation entropy for the amide rotation in Ac-AA-OMe has been reported for some proline analogues with additional polar groups,33 nonetheless, for some other polar proline analogues this value was also positive.34

Steric bulk of the N-acyl substituent

We then measured the amide rotation barriers for the full set of the prepared compounds in deuterium oxide medium (Fig. 7 and Table S4). The N-acetyl (2a–5a) and N-pivaloyl derivatives (2b–5b) feature the same high symmetry of the acyl moieties, while the steric bulk of the alkyl groups is significantly different. Due to the much higher steric size, the ground state is largely destabilized in the N-pivaloyl derivatives, and the overall difference from the N-acetyl compounds is about 15–21 kJ mol−1 (equivalent to a factor of 500–5000 at 298 K). At the same time, the bulky pivaloyl group excludes the possibility of the anti/exo or syn/endo transition state situations (proline nomenclature, see Fig. 1C), in which the tert-butyl group should move under the pyrrolidine ring. According to our semi-empirical modelling, these will be disfavored by about 11–12 kJ mol−1 in both N-pivaloyl derivatives 2b–3b, whereas in the N-acetyl compounds 2a–3a the disfavor is only by about 4–5 kJ mol−1. Finally, the N-acetylglycyl derivatives 2c–5c represent a symmetric N-peptidyl-substitution, and the rotation barriers here were reduced by about 3 kJ mol−1 (a factor of 3–4 at 298 K) compared to N-acetyl compounds 2a–5a. Notably, the amide rotation barriers found for the C-terminal methyl ester derivative of proline 5c are identical to the barriers found for the C-terminal peptidyl derivative AcGlyGlyProGlyGlyNH2,19 and from this we may conclude that the C-terminal ester adequately models the amide rotation in the polar medium such as water.
image file: c7ob01421j-f7.tif
Fig. 7 Amide rotation barriers of N-acyl derivatives. The values were determined in deuterium oxide solution (for AcGly-derivatives, buffered at pH 7 with potassium phosphate buffer) at 298 or 310 K. See ESI Table S4 for details.

Importantly, for all three types of tested compounds, the rotation barrier difference between the Pdc derivatives was about 8–10 kJ mol−1 (a factor of 25–55 at 298 K), with the barriers always lower for the mPdc derivative. This result confirms that the rotation barrier difference in this case originates from the transition state structures. It also highlights the fact that in both the syn/exo and anti/endo transition states shown in Fig. 1C, the alkyl group of the acetyl moiety moves outwards and cannot sterically interfere with the substituents in the pyrrolidine ring (see also Fig. 2). On the other hand, the carbonyl group of the amide fragment moves towards the pyrrolidine ring and may sterically interfere with a carboxymethyl group in rPdc derivatives and create polar interactions with the carboxymethyl groups in both rPdc and mPdc derivatives.

Polar effects

The polar interactions between the amide and carboxymethyl groups were then studied by comparison of the rotation barriers of the N-acetyl compounds (2a, 3a and 4a) in a set of nine NMR solvents (see Table S5). It is well known that the conjugation in the amide ground state is highly dependent on the solvent polarity;9,35 mechanistically, this is illustrated by the change in the amide resonance contributions [O[double bond, length as m-dash]C–N ↔ O–C[double bond, length as m-dash]N+]. The Reichardt–Dimroth parameter (ET) is a solvatochromic value that quantifies the ability of a solvent to separate intramolecular charges. Therefore, plotting rotation barriers in the EET coordinates produces positive slopes due to the differences in the ground state stabilities.16

While the experimental slopes (Fig. 8) for the rPdc and pyrrolidine derivatives (3a and 4a) were nearly identical (0.076 ± 0.006 and 0.075 ± 0.010, respectively), the slope for the mPdc derivative (2a) was notably larger (0.088 ± 0.008). This result illustrates differences in the polar effects of the transition states. We performed semi-empirical modelling of the amide rotation, and found that the overall molecular dipole follows the orientation of the amide carbonyl group C[double bond, length as m-dash]O in the pyrrolidine derivative 4a, and in the rPdc derivative 3a. In the latter compound the dipolar contribution of both carboxymethyl-moieties is mutually compensated for by the C2-symmetry of the residue (Fig. 9). As a result, the overall molecular dipole is defined by the orientation of the amide carbonyl group, and effectively, both 3a and 4a yield the same slope in the EET coordinates.


image file: c7ob01421j-f8.tif
Fig. 8 Amide rotation barriers for the N-acetyl derivatives 2a, 3a and 4a measured in different solvents by 1H EXSY NMR (at 298 or 310 K). For details see ESI Table S4.

image file: c7ob01421j-f9.tif
Fig. 9 Schematic representation of the dipolar effects during the amide rotation in N-acetylated compounds. log[thin space (1/6-em)]P values represent partitioning of the compounds between octan-1-ol and water at 298 K.

The orientation of the molecular dipole in 2a is more complex. The configuration of the two carboxymethyl substituents renders the compound more polar than 3a. For example, the Rf of mPdc derivatives are consistently lower compared with the equivalent rPdc derivatives (see the Experimental section). Higher polarity of the meso-diastereomer is also inferred from the octan-1-ol/water partitioning values as shown in Fig. 9. The amide rotation via the putative syn/exosyn/exo hybrid transition state partially compensates for the intramolecular dipole–dipole interaction in the transition state, causing the transition state to be relatively favored in the nonpolar solvents. As a result, the slope in the EET coordinates increases for 2a. Similar dipolar compensation can be expected for the amide rotation via the syn/exo transition state in peptidyl–prolyl fragments, and this effect might be enhanced due to a higher polarity of the amide fragment versus the esters reported here. As a result, the syn/exo transition state dominance should increase in less polar environments.

Conclusions

In summary, we described the amide rotation properties in N-acyl pyrrolidine-2,5-dicarboxylic acid derivatives, capped at the C-terminal sides as methyl esters. The meso diastereomer of this compound exhibited an amide rotation barrier of approximately 9 kJ mol−1 lower than that of the racemic diastereomer. This outcome was reproducible in three N-acyl derivatives with different degrees of steric bulk of the acyl-moieties to represent varying ground state energies.

This model system allowed us to demonstrate experimental features of the amide rotation relevant for peptidyl–prolyl groups: (1) the transitions are enthalpically driven and (2) differ in dipole–dipole compensation levels in the transition states and (3) the syn/exo transition scheme prevails in general over the anti/endo. These conclusions are consistent with the results of computational studies, which suggested the dominance of the syn/exo over the anti/endo transition state. At the same time, the other theoretically possible transition states, anti/exo and especially syn/endo, are expected to be disfavoured in peptide structures, as these would require the movement of a bulky upstream peptidyl-fragment under the pyrrolidine ring of proline.

Experimental section

Synthesis

N-Benzyl derivatives of Pdc were prepared starting from adipic acid as a mixture of two diastereomers.36 The resulting Bn-(MeO)Pdc-OMe compounds were separated on a silica gel column using hexane–ethyl acetate (4[thin space (1/6-em)]:[thin space (1/6-em)]1) as an eluent (Rf = 0.48 for rPdc and 0.27 for mPdc derivatives). The 1H NMR spectra exhibit a characteristic AB system for the diastereotopic benzyl CH2 group in the rPdc derivative, whereas this signal is a singlet in the mPdc derivative, where the CH2 moiety is not diastereotopic. 1H NMR (500 MHz, CDCl3), δ: Bn-(MeO)mPdc-OMe, 7.44–7.32 (m, 5H), 4.05 (s, 2H), 3.68 (s, 6H), 3.59 (br, 2H), 2.18 (m, 4H); Bn-(MeO)rPdc-OMe, 7.44–7.32 (m, 5H), 4.11 (d, J = 13 Hz, 1H), 3.92 (br d, J = 7 Hz, 2H), 3.91 (d, J = 13 Hz, 1H), 3.75 (s, 6H), 2.42 (m, 2H), 2.04 (m, 2H). The removal of the N-benzyl group was performed by stirring the substance with palladium on charcoal (10%) in methanol acidified with an equivalent amount of hydrochloric acid, under hydrogen pressure (40 atm), at 35 °C. The reaction was monitored by NMR. After the reaction was complete, the mixture was filtered, the solvent was removed under reduced pressure, and the residue was dissolved in water, filtered with activated charcoal and freeze-dried. The amino acid hydrochlorides were obtained as pinkish powders. 1H NMR (700 MHz, CD3OD), δ: HCl·H-(MeO)mPdc-OMe, 4.33 (t, J = 6 Hz, 2H), 3.85 (s, 6H), 2.40 (m, 2H), 2.15 (m, 2H); HCl·H-(MeO)rPdc-OMe, 4.22 (t, J = 6 Hz, 2H), 3.80 (s, 6H), 2.32 (m, 2H), 2.06 (m, 2H).
N-Acetyl compounds. N-Acetyl compounds were prepared as follows. A salt HCl·H-(MeO)Pdc-OMe (Pdc = mPdc or rPdc, 101 mg, 0.45 mmol) was stirred with acetic anhydride (0.4 ml, 4.2 mmol, 9 equiv.), triethylamine (0.2 ml, 1.4 mmol, 3 equiv.) and dimethylaminopyridine (5 mg, 0.04 mmol, 9 mol%) in a dichloromethane–tetrahydrofuran mixture (1.5 ml, 2[thin space (1/6-em)]:[thin space (1/6-em)]1) for 48 hours at room temperature. The solvent was gently removed under nitrogen gas flow, and the residue was dissolved in water (0.7 ml) and freeze-dried. The crude material was purified on a short silica gel column (about 10 g) using an ethyl acetate–methanol mixture (19[thin space (1/6-em)]:[thin space (1/6-em)]1) as an eluent (Rf = 0.49 for 2a and 0.63 for 3a).
Dimethyl(r,s)-1-acetylpyrrolidine-2,5-dicarboxylate (2a). Yellowish solid, yield 84 mg (0.37 mmol, 81%). 1H NMR (700 MHz, D2O), δ: 4.74 (dd, J = 7.0, 4.0 Hz, 1H), 4.44 (t, J = 7.5 Hz, 1H), 3.75 (s, 3H), 3.69 (s, 3H), 2.33–2.26 (m, 3H), 2.03 (s, 3H), 1.92 (m, 1H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 173.9 (C[double bond, length as m-dash]O), 173.8 (C[double bond, length as m-dash]O), 173.3 (C[double bond, length as m-dash]O), 61.2 (CH), 60.1 (CH), 53.1 (CH3O), 52.8 (CH3O), 29.4 (CH2), 27.7 (CH2), 21.2 (CH3). HMRS (ESI-orbitrap): calcd for [M + H]+ C10H16NO5 230.1023 Da, found 230.1028 Th. M.p. 60–65 °C.
Dimethyl(r,r)-1-acetylpyrrolidine-2,5-dicarboxylate (3a). White solid, yield 85 mg (0.37 mmol, 82%). 1H NMR (700 MHz, D2O), δ: 4.82 (d, J = 9.0 Hz, 1H), 4.54 (t, J = 8.8 Hz, 1H), 3.75 (s, 3H), 3.69 (s, 3H), 2.36 (m, 1H), 2.22 (m, 2H), 2.05 (m, 1H), 2.00 (s, 3H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 174.2 (C[double bond, length as m-dash]O), 174.0 (C[double bond, length as m-dash]O), 173.7 (C[double bond, length as m-dash]O), 61.1 (CH), 59.5 (CH), 53.4 (CH3O), 53.0 (CH3O), 29.2 (CH2), 27.0 (CH2), 21.1 (CH3). HMRS (ESI-orbitrap): calcd for [M + H]+ C10H16NO5 230.1023 Da, found 230.1030 Th. M.p. 95–100 °C.

The proline derivative Ac-Pro-OMe (5a) was prepared by mixing commercial racemic HCl·Pro-OMe with an equivalent amount of acetic anhydride in dichloromethane solution. The solvent was removed under reduced pressure, and the crude material was purified using a hexane–ethyl acetate gradient elution (from 1[thin space (1/6-em)]:[thin space (1/6-em)]1 to 0[thin space (1/6-em)]:[thin space (1/6-em)]1). The substance was obtained as clear oil, which crystallized upon long time storage at 4 °C. White solid, m.p. 35–40 °C. 1H NMR (700 MHz, D2O), δ (two rotamers, Ktrans/cis 4.95 ± 0.05 at 298 K): 4.62 (s-cis, dd, J = 8.8, 2.6 Hz) and 4.36 (s-trans, dd, J = 8.8, 4.6 Hz, 1H, α-CH), 3.72 (s-cis, s) and 3.67 (s, s-trans, 3H, CH3O), 3.59 and 3.55 (s-trans, two m) and 3.46 and 3.39 (s-cis, two m, 2H, δ-CH2), 2.28 and 2.15 (s-cis, two m) and 2.23 and 1.94 (s-trans, two m, 2H, β-CH2), 2.05 (s-trans, s) and 1.93 (s-cis, s, 3H, CH3C[double bond, length as m-dash]O), 1.95 (s-trans, m) and 1.90 and 1.80 (s-cis, two m, 2H, γ-CH2).

N-Acetyl pyrrolidine 4a was prepared as follows. Pyrrolidine (0.25 ml, 3 mmol) was mixed with acetic anhydride (0.29 ml, 3 mmol, 1 equiv.) in dichloromethane (5 ml) (CAUTION: vigorous reaction!). The mixture was stirred at room temperature for 30 min. Sodium carbonate (0.3 g) was added, and this mixture was shaken for about 15 hours at room temperature. The mixture was filtered, and the solvent was removed under reduced pressure to give 4a as a clear liquid. Yield 0.26 g (2.3 mmol, 75%). 1H NMR (500 MHz, D2O), δ: 3.41 (t, J = 7.0 Hz, 2H), 3.28 (t, J = 7.0 Hz, 2H), 1.97 (s, 3H), 1.86 (m, 2H), 1.79 (m, 2H).

N-Pivaloyl compounds. N-Pivaloyl compounds were prepared according to the procedure for the synthesis of 5b.37 A salt HCl·H-(MeO)Pdc-OMe (Pdc = mPdc or rPdc, 101 mg, 0.45 mmol) was mixed with pivaloyl chloride (70 μl, 0.57 mmol, 1.3 equiv.) in dichloromethane (1.5 ml), followed by the addition of triethylamine (150 μl, 1.08 mmol, 2.4 equiv.) and dimethylaminopyridine (5 mg, 0.04 mmol, 9 mol%). The mixture was stirred at room temperature for 4 hours. Then, it was washed with water (1 × 0.3 ml), 1 M aqueous hydrochloric acid (1 × 0.3 ml) and 1 M sodium hydrogencarbonate solution (1 × 0.3 ml), and subsequently dried over sodium sulphate, filtered and evaporated. The crude material was purified on a silica gel column (about 5 g), using a hexane–ethyl acetate mixture (1[thin space (1/6-em)]:[thin space (1/6-em)]1) as an eluent (Rf = 0.41 for 2b and 0.51 for 3b).
Dimethyl(r,s)-1-pivaloylpyrrolidine-2,5-dicarboxylate (2b). Clear oil, yield 80 mg (0.29 mmol, 66%). 1H NMR (700 MHz, D2O), δ: 5.03 (br d, J = 7 Hz, 1H), 4.43 (br t, J = 8 Hz, 1H), 3.72 (br s, 3H), 3.69 (br s, 3H), 2.26 (br m, 3H), 1.77 (br m, 1H), 1.13 (s, 9H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 180.7 (C[double bond, length as m-dash]O), 174.3 (C[double bond, length as m-dash]O), 174.0 (C[double bond, length as m-dash]O), 62.8 (CH), 60.8 (CH), 53.0 (CH3O), 52.6 (CH3O), 39.2 (C), 31.1 (CH2), 26.7 (CH3), 25.4 (CH2). HMRS (ESI-orbitrap): calcd for [M + H]+ C13H22NO5 272.1492 Da, found 272.1492 Th.
Dimethyl(r,r)-1-pivaloylpyrrolidine-2,5-dicarboxylate (3b). White solid, yield 86 mg (0.32 mmol, 70%). 1H NMR (700 MHz, D2O), δ: 5.14 (d, J = 8.5 Hz, 1H), 4.52 (d, J = 9.8 Hz, 1H), 3.74 (s, 3H), 3.67 (s, 3H), 2.38 (m, 1H), 2.19 (dd, J = 13.2, 6.6 Hz, 1H), 2.09 (m, 1H), 1.92 (dd, J = 13.5, 7.2 Hz, 1H), 1.12 (s, 9H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 180.4 (C[double bond, length as m-dash]O), 175.1 (C[double bond, length as m-dash]O), 174.8 (C[double bond, length as m-dash]O), 61.9 (CH), 60.9 (CH), 53.4 (CH3O), 52.8 (CH3O), 39.1 (C), 30.3 (CH2), 26.5 (CH3), 24.8 (CH2). HMRS (ESI-orbitrap): calcd for [M + H]+ C13H22NO5 272.1492 Da, found 272.1494 Th. M.p. 80–85 °C.

The synthesis of 5b was fully reproduced37 starting from 0.5 g of the racemic HCl·H-Pro-OMe (3.0 mmol, 1/10 scale of the reported synthesis). The product was a colorless oil. Yield 0.60 g (2.8 mmol, 92%). 1H NMR (700 MHz, D2O), δ (s-trans rotamer only, Ktrans/cis = 46 ± 2 at 298 K): 4.34 (dd, J = 8.5, 6.0 Hz, 1H, α-CH), 3.79 (dt, J = 10.6, 6.5 Hz, 1H, δ-CH), 3.74 (dt, J = 10.6, 6.7 Hz, 1H, δ-CH), 3.67 (s, 3H, CH3O), 2.14 (m, 1H, β-CH), 1.98 (m, 1H, γ-CH), 1.92 (m, 1H, γ-CH), 1.79 (m, 1H, β-CH), 1.17 (s, 9H, (CH3)3C).

Compound 4b was prepared as follows. Pyrrolidine (1 ml, 12.2 mmol) was mixed with triethylamine (3.4 ml, 24.4 mmol, 2 equiv.) in dichloromethane (20 ml). Pivaloyl chloride (1.6 ml, 13.0 mmol, 1.07 equiv.) was added dropwise at room temperature (CAUTION: vigorous reaction!), and the mixture was stirred for an additional 30 min. The solution was washed with water (1 × 5 ml), 1[thin space (1/6-em)]:[thin space (1/6-em)]2 aqueous hydrochloric acid (1 × 5 ml) and 1 M sodium hydrogen carbonate (1 × 5 ml); subsequently, it was dried over sodium sulphate, filtered and evaporated. Pure 4b was obtained as a colorless solid after crystallization from dichloromethane. Yield 1.80 g (11.6 mmol, 95%). 1H NMR (700 MHz, D2O), δ: 3.62 (t, J = 6.1 Hz, 2H), 3.34 (t, J = 6.7 Hz, 2H), 1.86 (m, 2H), 1.73 (m, 2H), 1.16 (s, 9H). M.p. 57–63 °C.

N-Acetylglycyl(N-aceturyl) compounds. N-Acetylglycyl(N-aceturyl) compounds were prepared under solution peptide coupling conditions. Aceturic acid (78 mg, 0.67 mmol, 1.3 equiv.) was mixed with benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP, 346 mg, 0.66 mmol, 1.3 equiv.) and diisopropylethylamine (120 μl, 0.69 mmol, 1.35 equiv.) in dichloromethane (2 ml). After about 10 min, the resulting solution was added to the mixture of HCl·H-(MeO)Pdc-OMe (Pdc = mPdc or rPdc, 113 mg, 0.51 mmol) with diisopropylethylamine (220 μl, 1.26 mmol, 2.5 equiv.) in dichloromethane (4 ml). The mixture was stirred for about 10 hours. It was then washed with 1[thin space (1/6-em)]:[thin space (1/6-em)]2 aqueous hydrochloric acid (1 × 0.5 ml) and 1 M sodium hydrogen carbonate (1 × 0.5 ml); subsequently, it was dried over sodium sulphate, filtered and evaporated. The resulting oil was extracted with water (1.5 ml). The extract was filtered through a short cation-exchange column (1.5 ml), and the elution was performed with water (about 5 ml). The aqueous fractions were collected and freeze-dried. Final purification was accomplished by silica gel chromatography (about 10 g) using an ethyl acetate–methanol gradient elution (19[thin space (1/6-em)]:[thin space (1/6-em)]1–4[thin space (1/6-em)]:[thin space (1/6-em)]1–0[thin space (1/6-em)]:[thin space (1/6-em)]1). The resulting compounds were colorless glassy materials and were slightly acidic. Therefore, the NMR samples in deuterium oxide were buffered with potassium phosphate buffer (70 mM, pH 7) and contained 35 mM of the analytes.
Dimethyl(r,s)-1-(acetylglycyl)pyrrolidine-2,5-dicarboxylate (2c). Yield 34 mg (0.12 mmol, 24%). 1H NMR (700 MHz, D2O), δ: 4.78 (dd, J = 7.9, 2.0 Hz, 1H), 4.47 (t, J = 7.8 Hz, 1H), 4.08 (d, J = 17.3 Hz, 1H), 3.93 (d, J = 17.1 Hz, 1H), 3.75 (s, 3H), 3.68 (s, 3H), 2.34–2.24 (m, 3H), 1.98 (s, 3H), 1.89 (m, 1H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 174.6 (C[double bond, length as m-dash]O), 173.8 (C[double bond, length as m-dash]O), 172.8 (C[double bond, length as m-dash]O), 170.5 (C[double bond, length as m-dash]O), 60.6 (CH), 59.9 (CH), 53.2 (CH3O), 52.9 (CH3O), 41.5 (NH-CH2), 29.4 (CH2), 27.2 (CH2), 21.6 (CH3). HRMS (ESI-orbitrap): calcd for [M + H]+ C12H19N2O6 287.1238 Da, found 230.1240 Th.
Dimethyl(r,r)-1-(acetylglycyl)pyrrolidine-2,5-dicarboxylate (3c). Yield 37 mg (0.13 mmol, 26%). 1H NMR (700 MHz, D2O), δ: 4.85 (d, J = 8.8 Hz, 1H), 4.56 (d, J = 9.4 Hz, 1H), 4.03 (d, J = 17.3 Hz, 1H), 3.84 (d, J = 17.3 Hz, 1H), 3.75 (s, 3H), 3.69 (s, 3H), 2.36 (m, 1H), 2.26 (dd, J = 13.6, 6.8 Hz, 1H), 2.17 (m, 1H), 2.04 (dd, J = 13.5, 7.0 Hz, 1H), 1.97 (s, 3H). 13C{1H} NMR (176 MHz, D2O), δ (all singlets): 174.5 (C[double bond, length as m-dash]O), 174.0 (C[double bond, length as m-dash]O), 173.5 (C[double bond, length as m-dash]O), 170.4 (C[double bond, length as m-dash]O), 60.0 (CH), 59.7 (CH), 53.5 (CH3O), 53.0 (CH3O), 41.5 (NH-CH2), 29.3 (CH2), 26.4 (CH2), 21.5 (CH3). HRMS (ESI-orbitrap): calcd for [M + H]+ C12H19N2O6 287.1238 Da, found 230.1239 Th.

The other two peptides 4c and 5c were prepared under analogous conditions, by employing N,N,N′,N‘-tetramethyl-O-(N-succinimidyl)uronium hexafluorophosphate (HSTU) as the coupling reagent. 4c, 1H NMR (700 MHz, D2O), δ: 3.93 (s, 2H), 3.40 (t, J = 6.9 Hz, 2H), 3.33 (t, J = 6.9 Hz, 2H), 1.98 (s, 3H), 1.90 (m, 2H), 1.80 (m, 2H). HRMS (ESI-orbitrap): calcd for [M + H]+ C8H15N2O2 171.1128 Da, found 171.1129 Th. 5c, 1H NMR (700 MHz, D2O), δ (s-trans rotamer only, Ktrans/cis = 6.15 ± 0.27 at 298 K): 4.41 (dd, J = 9.0, 4.4 Hz, 1H, α-CH), 4.03 (d, J = 17.3 Hz, 1H), 3.98 (d, J = 17.5 Hz, 1H), 3.68 (s, 3H, CH3O), 3.58 (m, 1H, δ-CH), 3.54 (m, 1H, δ-CH), 2.22 (m, 1H, β-CH), 1.98 (s, 3H), 1.97 (m, 2H, γ-CH2), 1.96 (m, 1H, β-CH). HRMS (ESI-orbitrap): calcd for [M + H]+ C10H17N2O4 229.1183 Da, found 229.1182 Th.

Physical chemistry

The NMR measurements were performed by 700 and 500 MHz 1H NMR detection. The variable temperature unit was calibrated using acidified methanol sample measurements.38
The pKa measurements. The pKa measurements were performed at 298 K as described.16 Buffer samples were taken at different pH values. Deuterium oxide was added (1/10 to the buffer volume), and the analyte (methyl ester hydrochloride of an amino acid) solution in a little amount of deuterium oxide was added 1–2 min prior to the 1H NMR measurement. The 1H NMR spectra were recorded using W5 water suppression pulse tray. The final analyte concentration was 0.5–1 mM. The measurements were performed in 10 mM phosphate buffer for pyrrolidine, 50 mM/60 mM phosphate/glycine buffer for HCl·H-Pro-OMe, and 20 mM/3 mM phosphate/citric buffer for HCl·H-(MeO)Pdc-OMe analytes. The standard error of the measured pKa values is ±0.10. For pyrrolidine the obtained value 11.41 is consistent with the previously reported value of 11.35.39
Partitioning. Partitioning was measured as follows. 5 mg of a compound was shaken with octan-1-ol (1.00 ml) and deionized water (1.00 ml) at 298 ± 2 K for 24 hours. Aliquots (250 μl) were added to identical NMR tubes and diluted with methanol-d4 (200 μl) for locking and shimming. The concentration of the samples was measured in single scan 90-degree pulse 1H NMR spectra recorded at 298 K. Only the 90-degree pulses and zero-phase values were re-adjusted between the measurements. The method is similar to that recently reported by Linclau et al. based on 19F NMR detection.40 Found log[thin space (1/6-em)]P values: 2a −0.74 ± 0.05, 3a −0.43 ± 0.03, 4a −0.20 ± 0.07, 5a −0.44 ± 0.05.
The amide rotation kinetics. The amide rotation kinetics were measured in 1H z-cross-relaxation experiments (NOESY/EXSY with z-gradients) as described.16,19 The analyte concentration in the NMR samples was 30–80 mM. Different mixing times (15 ms–5 s) between 2 and 5 were used for the exchange detection. The time domain in the direct dimension was 2048 points, whereas in the indirect dimension, this was set to yield sufficient resolution of the analysed resonances (160–512 points, resolution 5–30 Hz). Recycling delay was ≥2T1 of the analysed resonances. Note that the use of a high recycling delay is not necessary but improves the precision of the measurements. The exchange rate matrices were calculated using EXSYCalc (Mestrec), and the kinetic constants were converted to the activation energy values using the Eyring equation. The error of the activation energy values was calculated using the rate constant error and does not take into account the temperature calibration error. The results are summarized in the ESI in Table S1 (E for 2a–4a at different temperatures), Table S2 (E for 2c–4c at different temperatures), Table S3 (energetic terms for 2a–4a and 2c–4c), Table S4 (E in all compounds, D2O), and Table S5 (E for 2a–4a in different solvents).
Molecular modelling. Molecular modelling was performed using Scigress Modelling 3.2 (Fujitsu, Poland). The PM6-water algorithm was used for semi-empirical calculations.
X-ray diffraction. X-ray diffraction measurements were performed at 150 K. The R-factors for the calculated structures were 0.0257 (2a) and 0.0346 (3a).

Conflicts of interest

There is no conflict of interest to declare for this article.

Acknowledgements

VK acknowledges the DFG research group 1805 for a postdoctoral position and Synpeptide EU consortium for financial support. The authors are also grateful to Dr Marcie Jaffee (Atlanta, USA) for generous proof-reading of the manuscript.

Notes and references

  1. The proline/sodium symport is utilized by some organisms for the uptake of proline as a nutrient: (a) C.-C. Chen, T. Tsuchiya, Y. Yamane, J. M. Wood and T. H. Wilson, J. Membr. Biol., 1985, 84, 157–164 CrossRef CAS PubMed ; (b) S. Moses, T. Sinner, A. Zaprasis, N. Stöveken, T. Hoffmann, B. R. Belitsky, A. L. Sonenshein and E. Bremen, J. Bacteriol., 2012, 194, 745–758 CrossRef CAS PubMed ; (c) H. Jung, D. Hilger and M. Raba, Front. Biosci., 2012, 1, 745–759 CrossRef .
  2. (a) L. Szabados and A. Savouré, Trends Plant Sci., 2010, 15, 89–97 CrossRef CAS PubMed ; (b) I. Pérez-Arellano, F. Carmona-Álvarez, A. I. Martínez, J. Rodríguez-Díaz and J. Cervera, Protein Sci., 2010, 19, 372–382 Search PubMed .
  3. The ribosomal peptide bond formation for proline is slow compared to other amino acids: (a) M. Y. Pavlov, R. E. Watts, Z. Tan, V. W. Cornish, M. Ehrenberg and A. C. Forster, Proc. Natl. Acad. Sci. U. S. A., 2009, 106, 50–54 CrossRef CAS PubMed ; (b) L. K. Doerfel, I. Wohlgemuth, C. Kothe, F. Peske, H. Ulraub and M. V. Rodnina, Science, 2013, 339, 85–88 CrossRef CAS PubMed ; (c) L. K. Doerfel, I. Wohlgemuth, V. Kubyshkin, A. L. Starosta, D. N. Wilson, N. Budisa and M. V. Rodnina, J. Am. Chem. Soc., 2015, 137, 12997–13006 CrossRef CAS PubMed .
  4. (a) A. Farhat-Khemakhem, M. B. Ali, I. Boukhris, B. Khemachem, E. Maguin, S. Bejar and H. Chouayekh, Int. J. Biol. Macromol., 2013, 54, 9–15 CrossRef CAS PubMed ; (b) J. Huang, B. J. Jones and R. J. Kazlauskas, Biochemistry, 2015, 54, 4330–4341 CrossRef CAS PubMed ; (c) H. Yu, Y. Zhao, C. Guo, Y. Gan and H. Huang, Biochim. Biophys. Acta, Proteins Proteomics, 2015, 1854, 65–72 CrossRef CAS PubMed .
  5. Proline and its derivatives catalyse condensation reactions either as an organic additive: (a) S. Bertelsen and K. A. Jørgensen, Chem. Soc. Rev., 2009, 38, 2178–2189 RSC ; (b) M. H. Haindl, J. Hioe and R. M. Gschwind, J. Am. Chem. Soc., 2015, 137, 12835–12842 CrossRef CAS PubMed ; or this could also be an N-terminal proline residue in a short peptide: (c) J. Duschmalé, J. Wiest, M. Wiesner and H. Wennemers, Chem. Sci., 2013, 4, 1312–1318 RSC ; (d) T. Schnitzer, M. Wiesner, P. Krattiger, J. D. Revell and H. Wennemers, Org. Biomol. Chem., 2017, 15, 5877–5881 RSC ; or a protein: (e) M. Rahimi, E. M. Geertsema, Y. Miao, J.-Y. van der Meer, T. van den Bosch, P. de Haan, E. Zandvoort and G. J. Poelarends, Org. Biomol. Chem., 2017, 15, 2809–2816 RSC .
  6. Studies suggest a role of proline in the homochirality transfer from amino acids to sugars: J. E. Hein and D. G. Blackmond, Acc. Chem. Res., 2012, 45, 2045–2054 CrossRef CAS PubMed .
  7. (a) S. Kheria, R. V. Nair, A. S. Kotmale, P. R. Rajamohanan and G. J. Sanjayan, New J. Chem., 2015, 39, 3327–3332 RSC ; (b) N. D. Bansode, M. V. Sonar and K. N. Ganesh, Chem. Commun., 2016, 52, 4884–4887 RSC .
  8. I. E. Sampaio-Dias, C. A. D. Sousa, X. García-Mera, J. F. da Costa, O. Caamaño and J. E. Rodríguez-Borges, Org. Biomol. Chem., 2016, 14, 11065–11069 CAS .
  9. cis-trans-Isomerization in Biochemistry, ed. C. Dugave, Wiley, Weinheim, 2006 Search PubMed .
  10. (a) S. Fischer, S. Michnick and M. Karplus, Biochemistry, 1993, 32, 13830–13837 CrossRef CAS PubMed ; (b) C. Cox and T. Lectka, Acc. Chem. Res., 2000, 33, 849–858 CrossRef CAS PubMed ; (c) K. P. Lu, G. Finn, T. H. Lee and L. K. Nicholson, Nat. Chem. Biol., 2007, 3, 619–629 CrossRef CAS PubMed .
  11. G. Fischer, Chem. Soc. Rev., 2000, 29, 119–127 RSC .
  12. (a) G. Fischer, Angew. Chem., Int. Ed. Engl., 1994, 33, 1415–1436 CrossRef ; (b) S. F. Göthel and M. A. Marahiel, Cell. Mol. Life Sci., 1999, 55, 423–436 CrossRef PubMed .
  13. Examples of folding kinetics affected by the incorporation of fluoroprolines: in β2-microglobulin; (a) V. Yu. Torbeev and D. Hilvert, Proc. Natl. Acad. Sci. U. S. A., 2013, 110, 20051–20056 CrossRef CAS PubMed  in thioredoxin; (b) D. Roderer, R. Glockshuber and M. Rubini, ChemBioChem, 2015, 16, 2162–2166 CrossRef CAS PubMed .
  14. (a) V. Kubyshkin and N. Budisa, submitted; (b) D. Kern, M. Schutkowski and T. Drakenberg, J. Am. Chem. Soc., 1997, 119, 8403–8408 CrossRef CAS ; (c) G. Scherer, M. L. Kramer, M. Schutkowski, U. Reimer and G. Fischer, J. Am. Chem. Soc., 1998, 120, 5568–5574 CrossRef CAS ; (d) J. Zhang and M. W. German, Biopolymers, 2011, 95, 755–762 CrossRef CAS PubMed .
  15. N. W. Owens, C. Braun, J. D. O'Neil, K. Marat and F. Schweizer, J. Am. Chem. Soc., 2007, 129, 11670–11671 CrossRef CAS PubMed .
  16. V. Kubyshkin, P. Durkin and N. Budisa, New J. Chem., 2016, 40, 5209–5220 RSC .
  17. M. D. Shoulders and R. T. Raines, Annu. Rev. Biochem., 2009, 78, 929–958 CrossRef CAS PubMed .
  18. S. Busch, C. D. Bruce, C. Redfield, C. D. Lorenz and S. E. McLain, Angew. Chem., Int. Ed., 2013, 52, 13091–13095 CrossRef CAS PubMed .
  19. V. Kubyshkin and N. Budisa, Org. Biomol. Chem., 2017, 15, 619–627 CAS .
  20. (a) V. Somayaji and R. S. Brown, J. Org. Chem., 1986, 51, 2676–2686 CrossRef CAS ; (b) A. J. Kirby, I. V. Komarov, P. D. Wothers and N. Feeder, Angew. Chem., Int. Ed., 1998, 37, 785–786 CrossRef CAS ; (c) A. J. Kirby, I. V. Komarov and N. Feeder, J. Am. Chem. Soc., 1998, 120, 7101–7102 CrossRef CAS ; (d) J. Clayden and W. J. Moran, Angew. Chem., Int. Ed., 2006, 45, 7118–7120 CrossRef CAS PubMed ; (e) I. V. Komarov, S. Yanik, A. Yu. Ishchenko, J. E. Davies, J. M. Goodman and A. J. Kirby, J. Am. Chem. Soc., 2015, 137, 926–930 CrossRef CAS PubMed ; (f) R. Szostak, J. Aubé and M. Szostak, J. Org. Chem., 2015, 80, 7905–7927 CrossRef CAS PubMed ; (g) M. Liniger, D. G. VanderVelde, M. K. Takase, M. Shahgholi and B. M. Stolz, J. Am. Chem. Soc., 2016, 138, 969–974 CrossRef CAS PubMed .
  21. The early computational analysis of AcProNHMe suggested that the syn/exo and anti/endo transition states are most favoured, with the energy difference of about 6 kJ mol−1, whereas no saddle point was found for the syn/endo state, due to the steric repulsion between the N- and C-terminal groups in this transition state: S. Fischer, R. L. Dunbrack Jr. and M. Karplus, J. Am. Chem. Soc., 1994, 116, 11931–11937 CrossRef CAS .
  22. S. Adachi, N. Kumagai and M. Shibasaki, Chem. Sci., 2017, 8, 85–90 RSC .
  23. See, for example: (a) Y. K. Kang and H. Y. Choi, Biophys. Chem., 2004, 111, 135–142 CrossRef CAS PubMed ; (b) A. E. Aliev, S. Bhandal and D. Courtier-Murias, J. Phys. Chem. A, 2009, 113, 10858–10865 CrossRef CAS PubMed ; (c) J. Chen, S. A. Edwards, F. Gräter and C. Baldauf, J. Phys. Chem. B, 2012, 116, 9346–9351 CrossRef CAS PubMed .
  24. E. Vöhringer-Martinez, F. Duarte and A. Toro-Labbé, J. Phys. Chem. B, 2012, 116, 12972–12979 CrossRef PubMed .
  25. A. Y. Mercedez-Camacho, A. B. Mullins, M. D. Mason, G. G. Xu, B. J. Mahoney, X. Wang, J. W. Peng and F. A. Etzkorn, Biochemistry, 2013, 52, 7707–7713 CrossRef PubMed .
  26. C. Cox and T. Lectka, J. Am. Chem. Soc., 1998, 120, 10660–10668 CrossRef CAS .
  27. The structures have been deposited in Cambridge Crystallographic Data Centre under the deposition numbers: 2a – CCDC 1554475, 3a – CCDC 1554476..
  28. M. Cai, Y. Huang, J. Liu and R. Krishnamoorthi, J. Biomol. NMR, 1995, 6, 123–128 CrossRef CAS PubMed .
  29. R. W. Newberry and R. T. Raines, Acc. Chem. Res., 2017 DOI:10.1021/acs.accounts.7b00121 .
  30. C. Siebler, B. Maryasin, M. Kuemin, R. S. Erdmann, C. Rigling, C. Grünenfelder, C. Ochsenfeld and H. Wennemers, Chem. Sci., 2015, 6, 6725–6730 RSC .
  31. C. Renner, S. Alefelder, J. H. Bae, N. Budisa, R. Huber and L. Moroder, Angew. Chem., Int. Ed., 2001, 40, 923–925 CrossRef CAS PubMed .
  32. Somehow higher activation entropy values have been reported for 5a in E. S. Eberhardt, N. Panasik Jr. and R. T. Raines, J. Am. Chem. Soc., 1996, 118, 12261–12266 CrossRef CAS PubMed .
  33. 3-Fluoroprolines; (a) C. A. Thomas, E. R. Talaty and J. G. Bann, Chem. Commun., 2009, 3366–3368 RSC ; and 4-trifluoromethylproline; (b) V. Kubyshkin, S. Afonin, S. Kara, N. Budisa, P. K. Mykhailiuk and A. S. Ulrich, Org. Biomol. Chem., 2015, 13, 3171–3181 RSC .
  34. 4-Fluoroproline, see ref. 31; 4-hydroxyproline and its O-glycosylated derivatives, see ref. 15, 4-azidoproline see L.-S. Sonntag, PhD Thesis, Basel University, 2005 .
  35. (a) E. S. Eberhardt, S. N. Loh, A. P. Hinck and R. T. Raines, J. Am. Chem. Soc., 1992, 114, 5437–5439 CrossRef CAS PubMed ; (b) C. Cox and T. Lectka, J. Org. Chem., 1998, 63, 2426–2427 CrossRef CAS PubMed .
  36. V. S. Kubyshkin, P. K. Mykhailiuk and I. V. Komarov, Tetrahedron Lett., 2007, 48, 4061–4063 CrossRef CAS .
  37. D. N. Reddy, R. Thirupathi, S. Tumminakatti and E. N. Prabhakaran, Tetrahedron Lett., 2012, 53, 4413–4417 CrossRef CAS .
  38. A. L. Van Geet, Anal. Chem., 1970, 42, 679–680 CrossRef CAS .
  39. V. Frenna, N. Vivona, G. Consiglio and D. Spinelli, J. Chem. Soc., Perkin Trans. 2, 1985, 1865–1868 RSC .
  40. B. Linclau, Z. Wang, G. Compain, V. Paumelle, C. Q. Fontenelle, N. Wells and A. Weymouth-Wilson, Angew. Chem., Int. Ed., 2016, 55, 674–678 CrossRef CAS PubMed .

Footnotes

The article is dedicated to the memory of Prof. Yuriy Kholin (1962–2017), who nurtured a generation of young talented Ukrainian chemists.
Electronic supplementary information (ESI) available: Tables S1–S5, copies of the NMR spectra for compounds. CCDC 1554475 and 1554476. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7ob01421j

This journal is © The Royal Society of Chemistry 2017